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  1. No Access

    Article

    Analysis of the Ovarian Marker Genes Expression Revealed the Antagonistic Effects of Serotonin and Androstenedione on the Functional State of Mouse Granulosa Cells in Primary Culture

    Primary culture of granulosa cells is a prerequisite for a complete study of the normal functioning of the ovary and its pathologies. In this work, we selected the optimal protocol for obtaining a primary cult...

    N. M. Alyoshina, V. R. Rousanova… in Russian Journal of Developmental Biology (2023)

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    Article

    The Serotonin System in Mammalian Oogenesis

    Transmitters, particularly serotonin, not only have the classical function of transmitting nerve impulses, but also take part in a whole series of regulatory processes throughout ontogeny, including those occu...

    Yu. B. Shmukler, N. M. Alyoshina, L. A. Malchenko… in Neuroscience and Behavioral Physiology (2022)

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    Article

    Oocyte-Mediated Effect of Serotonin on the Functional Status of Granulosa Cells

    In the mammalian ovary, serotonin has all the components necessary for its signaling function and affects the functional activity of granulosa cells. At the same time, the accumulation of serotonin with the he...

    D. A. Nikishin, Y. V. Khramova, N. M. Alyoshina… in Russian Journal of Developmental Biology (2021)

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    Article

    Synthesis and Membrane Transport of Serotonin in the Develo** Ovarian Follicle of Mouse

    Using RT-PCR, we showed the presence of Tph1 mRNA in follicular cells and Tph2 mRNA in oocytes isolated from primary multilayer ovarian follicles of mouse and the absence of Ddc expression, which indicates that s...

    D. A. Nikishin, N. M. Alyoshina, Y. B. Shmukler in Doklady Biochemistry and Biophysics (2018)