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Open AccessA bistable inhibitory optoGPCR for multiplexed optogenetic control of neural circuits
Information is transmitted between brain regions through the release of neurotransmitters from long-range projecting axons. Understanding how the activity of such long-range connections contributes to behavior...
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Article
Open AccessDevelopment of a genetically encoded sensor for probing endogenous nociceptin opioid peptide release
Nociceptin/orphanin-FQ (N/OFQ) is a recently appreciated critical opioid peptide with key regulatory functions in several central behavioral processes including motivation, stress, feeding, and sleep. The func...
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Article
Targeting Nociceptin/Orphanin FQ receptor to rescue cognitive symptoms in a mouse neuroendocrine model of chronic stress
Chronic stress causes cognitive deficits, such as impairments in episodic-like hippocampus-dependent memory. Stress regulates an opioid-related neuropeptide named Nociceptin/Orphanin FQ (N/OFQ), the ligand of ...
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Article
Open AccessInduction of a torpor-like hypothermic and hypometabolic state in rodents by ultrasound
Torpor is an energy-conserving state in which animals dramatically decrease their metabolic rate and body temperature to survive harsh environmental conditions. Here, we report the noninvasive, precise and saf...
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Article
Pain induces adaptations in ventral tegmental area dopamine neurons to drive anhedonia-like behavior
The persistence of negative affect in pain leads to co-morbid symptoms such as anhedonia and depression—major health issues in the United States. The neuronal circuitry and contribution of specific cellular po...
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Author Correction: Ventral tegmental area GABAergic inhibition of cholinergic interneurons in the ventral nucleus accumbens shell promotes reward reinforcement
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Article
An endogenous opioid circuit determines state-dependent reward consumption
µ-Opioid peptide receptor (MOPR) stimulation alters respiration, analgesia and reward behaviour, and can induce substance abuse and overdose1–3. Despite its evident importance, the endogenous mechanisms for MOPR ...
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Article
Ventral tegmental area GABAergic inhibition of cholinergic interneurons in the ventral nucleus accumbens shell promotes reward reinforcement
The long-range GABAergic input from the ventral tegmental area (VTA) to the nucleus accumbens (NAc) is relatively understudied, and therefore its role in reward processing has remained unknown. In the present ...
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Article
Wireless optofluidic brain probes for chronic neuropharmacology and photostimulation
Both in vivo neuropharmacology and optogenetic stimulation can be used to decode neural circuitry, and can provide therapeutic strategies for brain disorders. However, current neuronal interfaces hinder long-t...
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NOP Receptor Signaling Cascades
The nociceptin/orphanin FQ (N/OFQ) peptide (NOP) receptor is a G protein-coupled receptor with wide distribution throughout the peripheral and central nervous system. Similar to other opioid receptors, NOP rec...
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Article
Open AccessNicotine aversion is mediated by GABAergic interpeduncular nucleus inputs to laterodorsal tegmentum
Nicotine use can lead to dependence through complex processes that are regulated by both its rewarding and aversive effects. Recent studies show that aversive nicotine doses activate excitatory inputs to the i...
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Article
Polymorphic computation in locus coeruleus networks
Physiological and optogenetic dissection of discrete locus coeruleus neuronal populations reveals a functional disassociation, with heterogeneous engagement of locus coeruleus neurons in either fear learning o...
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Preparation and implementation of optofluidic neural probes for in vivo wireless pharmacology and optogenetics
This protocol extension describes the fabrication of optofluidic neural probes and implantation for advanced in vivo pharmacology and optogenetics in freely moving rodents.
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Chemogenetic and Optogenetic Activation of Gαs Signaling in the Basolateral Amygdala Induces Acute and Social Anxiety-Like States
Anxiety disorders are debilitating psychiatric illnesses with detrimental effects on human health. These heightened states of arousal are often in the absence of obvious threatening cues and are difficult to t...
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Architectural Representation of Valence in the Limbic System
In order to thrive, animals must be able to recognize aversive and appetitive stimuli within the environment and subsequently initiate appropriate behavioral responses. This assignment of positive or negative ...
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Soft, stretchable, fully implantable miniaturized optoelectronic systems for wireless optogenetics
Optogenetic studies of the peripheral nervous system and spinal cord are enabled by soft, wirelessly powered biocompatible devices.
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Article
Open AccessOptodynamic simulation of β-adrenergic receptor signalling
Optogenetics has provided a revolutionary approach to dissecting biological phenomena. However, the generation and use of optically active GPCRs in these contexts is limited and it is unclear how well an opsin...
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Article
Open AccessPhoto-activatable Cre recombinase regulates gene expression in vivo
Techniques allowing precise spatial and temporal control of gene expression in the brain are needed. Herein we describe optogenetic approaches using a photo-activatable Cre recombinase (PA-Cre) to stably modif...
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Article
Functional Selectivity at GPCRs: New Opportunities in Psychiatric Drug Discovery
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Fabrication and application of flexible, multimodal light-emitting devices for wireless optogenetics
The rise of optogenetics provides unique opportunities to advance materials and biomedical engineering, as well as fundamental understanding in neuroscience. This protocol describes the fabrication of optoelec...
