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Article
Open AccessAb-GALFA, A bioassay for insect gall formation using the model plant Arabidopsis thaliana
Insect galls are abnormal plant organs formed by gall-inducing insects to provide shelter and nutrients for themselves. Although insect galls are spatialized complex structures with unique shapes and function...
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Article
Author Correction: PtdIns(3,5)P2 mediates root hair shank hardening in Arabidopsis
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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Physiological Functions of Phosphoinositide-Modifying Enzymes and Their Interacting Proteins in Arabidopsis
The integrity of cellular membranes is maintained not only by structural phospholipids such as phosphatidylcholine and phosphatidylethanolamine, but also by regulatory phospholipids, phosphatidylinositol phosp...
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Article
PtdIns(3,5)P2 mediates root hair shank hardening in Arabidopsis
Root hairs elongate by tip growth and simultaneously harden the shank by constructing the inner secondary cell wall layer. While much is known about the process of tip growth1, almost nothing is known about the m...
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Article
Nuclear membrane localization during pollen development and apex-focused polarity establishment of SYP124/125 during pollen germination in Arabidopsis thaliana
Establishment of apex-polarity.
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Article
Expression of a rice glutaredoxin in aleurone layers of develo** and mature seeds: subcellular localization and possible functions in antioxidant defense
A rice glutaredoxin isoform (OsGrxC2;2) with antioxidant capacity is expressed abundantly in seed tissues and is localized to storage vacuoles in aleurone layers in develo** and mature seeds.
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Article
Open AccessSplit luciferase complementation assay to detect regulated protein-protein interactions in rice protoplasts in a large-scale format
The rice interactome, in which a network of protein-protein interactions has been elucidated in rice, is a useful resource to identify functional modules of rice signal transduction pathways. Protein-protein i...
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Article
Open AccessIncrease methylmercury accumulation in Arabidopsis thaliana expressing bacterial broad-spectrum mercury transporter MerE
The bacterial merE gene derived from the Tn21 mer operon encodes a broad-spectrum mercury transporter that governs the transport of methylmercury and mercuric ions across bacterial cytoplasmic membranes, and this...
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Article
Requirement of vesicle-associated membrane protein 721 and 722 for sustained growth during immune responses in Arabidopsis
Extracellular immune responses to ascomycete and oomycete pathogens in Arabidopsis are dependent on vesicle-associated secretion mediated by the SNARE proteins PEN1 syntaxin, SNAP33 and endomembrane-resident V...
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Article
Expression of the bacterial heavy metal transporter MerC fused with a plant SNARE, SYP121, in Arabidopsis thaliana increases cadmium accumulation and tolerance
The bacterial merC gene from the Tn21-encoded mer operon is a potential molecular tool for improving the efficiency of metal phytoremediation. Arabidopsis SNARE molecules, including SYP111, SYP121, and AtVAM3 (SY...
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Chapter
The Role of RAB GTPases and SNARE Proteins in Plant Endocytosis and Post-Golgi Trafficking
Each membrane trafficking pathway involves several evolutionarily conserved key molecules, including RAB GTPases and SNARE proteins. Distinct sets of RAB and SNARE molecules regulate tethering and fusion of th...
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Article
A membrane trafficking pathway regulated by the plant-specific RAB GTPase ARA6
Plants possess a unique repertoire of SNARE and RAB GTPase proteins that regulate membrane trafficking events. The plant-specific RAB protein ARA6 regulates the formation of a specific SNARE complex to modulat...
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Article
Luminescence detection of SNARE–SNARE interaction in Arabidopsis protoplasts
Membrane associated proteins SNAREs (soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptors) provide the minimal fusion machinery necessary for cellular vesicles to fuse to target organell...
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Chapter
SNAREs in Plant Endocytosis and the Post-Golgi Traffic
In eukaryotic cells, the transport vesicles carry various cargo proteins from a donor compartment to a target compartment, and discharge the cargo into the target compartment by fusing with the membrane ...
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Article
Pollen-specific regulation of vacuolar H+-PPase expression by multiple cis-acting elements
We dissected the regulatory region of the AVP1 gene encoding the vacuolar H+-pyrophosphatase (V-PPase) of Arabidopsis thaliana by using a GUS-reporter assay system. The cloned 1.4 kb 5′-regulatory region in the G...