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  1. Article

    Open Access

    Increase methylmercury accumulation in Arabidopsis thaliana expressing bacterial broad-spectrum mercury transporter MerE

    The bacterial merE gene derived from the Tn21 mer operon encodes a broad-spectrum mercury transporter that governs the transport of methylmercury and mercuric ions across bacterial cytoplasmic membranes, and this...

    Yuka Sone, Ryosuke Nakamura, Hidemitsu Pan-Hou, Masa H Sato, Tomoo Itoh… in AMB Express (2013)

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  2. Article

    Open Access

    Mercurial-resistance determinants in Pseudomonas strain K-62 plasmid pMR68

    We report the complete nucleotide sequence of plasmid pMR68, isolated from Pseudomonas strain K-62, two plasmids contribute to broad-spectrum mercury resistance and that the mer operon from one of them (pMR26) ha...

    Yuka Sone, Yusuke Mochizuki, Keita Koizawa, Ryosuke Nakamura… in AMB Express (2013)

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  3. No Access

    Article

    Expression of the bacterial heavy metal transporter MerC fused with a plant SNARE, SYP121, in Arabidopsis thaliana increases cadmium accumulation and tolerance

    The bacterial merC gene from the Tn21-encoded mer operon is a potential molecular tool for improving the efficiency of metal phytoremediation. Arabidopsis SNARE molecules, including SYP111, SYP121, and AtVAM3 (SY...

    Masako Kiyono, Yumiko Oka, Yuka Sone, Michitaka Tanaka, Ryosuke Nakamura… in Planta (2012)

  4. No Access

    Article

    Development of a transgenic tobacco plant for phytoremediation of methylmercury pollution

    To develop the potential of plant for phytoremediation of methylmercury pollution, a genetically engineered tobacco plant that coexpresses organomercurial lyase (MerB) with the ppk-specified polyphosphate (polyP)...

    Takeshi Nagata, Hirofumi Morita… in Applied Microbiology and Biotechnology (2010)

  5. No Access

    Article

    Engineering expression of the heavy metal transporter MerC in Saccharomyces cerevisiae for increased cadmium accumulation

    The merC gene from the Tn21-encoded mer operon has potential uses as a molecular tool for bioremediation. It was overexpressed as the fusion proteins MerC-Sso1p or MerC-Vam3p in Saccharomyces cerevisiae. Green fl...

    Masako Kiyono, Kiyomi Miyahara, Yuka Sone… in Applied Microbiology and Biotechnology (2010)

  6. No Access

    Article

    Engineering expression of bacterial polyphosphate kinase in tobacco for mercury remediation

    To develop the potential of plants to sequester and accumulate mercurials from the contaminated sites, we engineered a tobacco (Nicotiana tabacum) plant to express a bacterial ppk gene, encoding polyphosphate kin...

    Takeshi Nagata, Masako Kiyono, Hidemitsu Pan-Hou in Applied Microbiology and Biotechnology (2006)

  7. No Access

    Article

    Organomercurial resistance determinants in Pseudomonas K-62 are present on two plasmids

    Pseudomonas strain K-62 was found to contain six plasmids. A mutant derivative cured of the 26-kb plasmid showed a higher sensitivity to mercurials; however, the strain was still able to volatilize them. Loss of...

    Masako Kiyono, Tomoko Omura, Hiroyuki Fujimori… in Archives of Microbiology (1995)