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Protocol
Nanodiscs as a New Tool to Examine Lipid–Protein Interactions
The interactions between lipids and proteins are one of the most fundamental processes in living organisms, responsible for critical cellular events ranging from replication, cell division, signaling, and move...
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Article
Cytochrome P450 Genes from the Sacred Lotus Genome
Cytochrome P450 monooxygenases (P450s) in the sacred lotus (Nelumbo nucifera) genome have been identified and named according to systematic P450 nomenclatures. Comparisons of these sequences with those in the pap...
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Article
Structure and Function of Cytochrome P450S in Insect Adaptation to Natural and Synthetic Toxins: Insights Gained from Molecular Modeling
Over evolutionary time, insect herbivores have adapted to the presence of natural toxins and more recently to synthetic insecticides in or on the plants they consume. Biochemical analyses and molecular modelin...
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Article
Open AccessGenome of the long-living sacred lotus (Nelumbo nucifera Gaertn.)
Sacred lotus is a basal eudicot with agricultural, medicinal, cultural and religious importance. It was domesticated in Asia about 7,000 years ago, and cultivated for its rhizomes and seeds as a food crop. It ...
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Article
Rice P450 reductases differentially affect P450-mediated metabolism in bacterial expression systems
We describe cloning and characterization of three rice (Oryza sativa) NADPH-cytochrome P450 reductases (OsCPRs; E.C.1.6.2.4) that are potential donors to plant P450s, including tryptamine 5-hydroxylase (T5H) in s...
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Protocol
Nanodiscs as a New Tool to Examine Lipid–Protein Interactions
Nanodiscs are self-assembled discoidal fragments of lipid bilayers 8–16 nm in diameter, stabilized in solution by two amphipathic helical scaffold proteins. As stable and highly soluble membrane mimetics with ...
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Article
Genome of papaya, a fast growing tropical fruit tree
Papaya is a major fruit crop in tropical and subtropical regions worldwide. It has long been recognized as a nutritious and healthy fruit rich in vitamins A and C. Its small genome, unique aspects of nascent s...
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Article
Open AccessEvolutionary Toxicogenomics: Diversification of the Cyp12d1 and Cyp12d3 Genes in Drosophila Species
Gene duplication and divergence are overwhelmingly considered to be the primary mechanisms by which cytochrome P450 monooxygenases (P450s) have radiated into a large and diverse gene superfamily. To address ho...
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Article
VITAL NMR: using chemical shift derived secondary structure information for a limited set of amino acids to assess homology model accuracy
Homology modeling is a powerful tool for predicting protein structures, whose success depends on obtaining a reasonable alignment between a given structural template and the protein sequence being analyzed. In...
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Article
Arabidopsis CYP72C1 is an atypical cytochrome P450 that inactivates brassinosteroids
Cytochrome P450 monooxygenases (P450s) are a diverse family of proteins that have specialized roles in secondary metabolism and in normal cell development. Two P450s in particular, CYP734A1 and CYP72C1, have b...
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Article
Comparative Toxicity of Mycotoxins to Navel Orangeworm (Amyelois transitella) and Corn Earworm (Helicoverpa zea)
Mycotoxins, such as aflatoxins and ochratoxins, are widely distributed in nature and are frequently problematic crop contaminants that cause millions of dollars of annual losses in the United States. Insect in...
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Article
Enhanced Toxicity and Induction of Cytochrome P450s Suggest a Cost of “Eavesdrop**” in a Multitrophic Interaction
The inducibility of cytochrome P450 monooxygenases (P450s) and other xenobiotic-metabolizing enzymes is thought to reflect material and energy costs of biosynthesis. Efforts to detect such costs of detoxificat...
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Article
Ecological Significance of Induction of Broad-Substrate Cytochrome P450s by Natural and Synthetic Inducers in Helicoverpa zea
The polyphagous corn earworm Helicoverpa zea relies on cytochrome P450 monooxygenases with broad substrate specificities to cope with the wide diversity of phytochemicals it encounters among its numerous host pla...
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Article
Comparison of Cytochrome P450 Genes from Six Plant Genomes
Plants depend on cytochrome P450 (CYP) enzymes for nearly every aspect of their biology. In several sequenced angiosperms, CYP genes constitute up to 1% of the protein coding genes. The angiosperm sequence div...
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Article
Open AccessThe draft genome of the transgenic tropical fruit tree papaya (Carica papaya Linnaeus)
In the early 1990s an outbreak of papaya ringspot virus (PRSV) in the papaya groves in the Puna district of Hawaii caused severe damage to an important crop. Since then, the planting of two transgenic cultivar...
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Article
Allelochemical Induction of Cytochrome P450 Monooxygenases and Amelioration of Xenobiotic Toxicity in Helicoverpa zea
Polyphagous herbivores encounter allelochemicals as complex mixtures in their host plants, and the toxicity of an individual compound may be influenced by the chemical matrix in which it is encountered. Certai...
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Article
Toxicity of Aflatoxin B1 to Helicoverpa zea and Bioactivation by Cytochrome P450 Monooxygenases
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Article
Toxicity of Aflatoxin B1 to Helicoverpa zea and Bioactivation by Cytochrome P450 Monooxygenases
Infestation of corn (Zea mays) by corn earworm (Helicoverpa zea) predisposes the plant to infection by Aspergillus fungi and concomitant contamination with the carcinogenic mycotoxin aflatoxin B1 (AFB1). Although...
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Article
Open AccessArabidopsis cytochrome P450s through the looking glass: a window on plant biochemistry
Annotation of the genome sequence of Arabidopsis thaliana has identified a diverse array of 245 full-length cytochrome P450 monooxygenase (P450) genes whose known functions span the synthetic gamut from critical ...
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Article
Heterologous expression and strategies for encapsulation of membrane-localized plant P450s
Heterologous expression of plant P450 proteins is critical for functional definitions of their enzymatic activities as well as for producing natural products whose biosyntheses involve P450s. Over the past dec...
