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Article
Open AccessDoes a lack of vaccine side effects correlate with reduced BNT162b2 mRNA vaccine response among healthcare workers and nursing home residents?
The BNT162b2 SARS-CoV-2 mRNA vaccination has mitigated the burden of COVID-19 among residents of long-term care facilities considerably, despite being excluded from the vaccine trials. Data on reactogenicity (...
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Article
Blastocyst Vitrification and Trophectoderm Biopsy Cumulatively Alter Embryonic Gene Expression in a Mouse Model
Although embryo vitrification has been used extensively in human assisted reproductive technology (ART) and animal models, epidemiologic evidence and randomized controlled trials suggest differences in pregnan...
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Article
Open AccessSex and age bias viral burden and interferon responses during SARS-CoV-2 infection in ferrets
SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) hospitalizations and deaths disportionally affect males and older ages. Here we investigated the impact of male sex and age comparing sex-matched or...
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Article
Open AccessA follicular regulatory Innate Lymphoid Cell population impairs interactions between germinal center Tfh and B cells
Innate Lymphoid Cells (ILCs) are immune cells typically found on mucosal surfaces and in secondary lymphoid organs where they regulate the immune response to pathogens. Despite their key role in the immune res...
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Article
Open AccessDrug perturbation gene set enrichment analysis (dpGSEA): a new transcriptomic drug screening approach
In this study, we demonstrate that our modified Gene Set Enrichment Analysis (GSEA) method, drug perturbation GSEA (dpGSEA), can detect phenotypically relevant drug targets through a unique transcriptomic enri...
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Article
Open AccessRecommendations for Selection and Characterization of Protein Biomarker Assay Calibrator Material
As biomarkers continue to become an integral part of drug development and decision-making, there are increased expectations for reliable and quantitative assays. Protein biomarker assay results are directly in...
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Article
Current Trends in Ligand Binding Real-Time Measurement Technologies
Numerous advances in ligand binding assay (LBA) real-time measurement technologies have been made within the last several years, ranging from the development of novel platforms to drive technology expansion to...
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Article
Open AccessA CD4+T cell antagonist epitope down-regulates activating signaling proteins, up-regulates inhibitory signaling proteins and abrogates HIV-specific T cell function
CD4+ T cells are critically important in HIV infection, being both the primary cells infected by HIV and likely playing a direct or indirect role in hel** control virus replication. Key areas of interest in HIV...
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Article
Lymph node T cell responses predict the efficacy of live attenuated SIV vaccines
Live attenuated SIV vaccines protect nonhuman primates from infection with pathogenic wild-type SIV, but the crucial mechanisms have not been clear. In this issue, Louis Picker and colleagues show that protect...
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Bead-Based Flow Cytometric Assays: A Multiplex Assay Platform with Applications in Diagnostic Microbiology
Researchers have focused on develo** specific assays for conclusively identifying and measuring the levels of bacteria, fungi, protozoa, viruses (microbes), and their associated products (biomarkers) that ca...
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Article
Interleukin-4
The nonobese diabetic (NOD) mouse spontaneously develops a diabetic syndrome that closely resembles human insulin-dependent diabetes mellitus (IDDM). T cell-mediated destruction of pancreatic cells may result ...
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Article
Direct transfer of a foreign MHC gene into human melanoma alters T cell receptor Vβ usage by tumor-infiltrating lymphocytes
The direct introduction of foreign genes into tumors shows promise as a therapeutic modality to enhance tumor immunogenicity. Hence, melanoma nodules were directly injected with a vector encoding an allogenei...
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Article
Diverse manifestations of tumorigenicity and immunogenicity displayed by the poorly immunogenic B16-BL6 melanoma transduced with cytokine genes
We evaluated the in vivo response to the poorly immunogenic B16-BL6 (BL6) murine melanoma genetically altered to secrete interleukin-2 (IL-2), IL-4, interferon γ (IFNγ) and granulocyte/macrophage-colony-stimu...
