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Article
Open AccessCitrate synthase variants improve yield of acetyl-CoA derived 3-hydroxybutyrate in Escherichia coli
The microbial chiral product (R)-3-hydroxybutyrate (3-HB) is a gateway to several industrial and medical compounds. Acetyl-CoA is the key precursor for 3-HB, and several native pathways compete with 3-HB produ...
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Article
Pretreatment and Detoxification of Acid-Treated Wood Hydrolysates for Pyruvate Production by an Engineered Consortium of Escherichia coli
The biorefinery concept makes use of renewable lignocellulosic biomass to produce commodities sustainably. A synthetic microbial consortium can enable the simultaneous utilization of sugars such as glucose and...
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Article
Characterization of the Furfural and 5-Hydroxymethylfurfural (HMF) Metabolic Pathway in the Novel Isolate Pseudomonas putida ALS1267
The genes involved in the aerobic bacterial metabolism of furfural and 5-hydroxymethylfurfural (HMF) have been characterized in two species, Pseudomonas putida Fu1 and Cupriavidus basilensis HMF14. A third furan-...
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Article
Open AccessRemoval of aromatic inhibitors produced from lignocellulosic hydrolysates by Acinetobacter baylyi ADP1 with formation of ethanol by Kluyveromyces marxianus
Lignocellulosic biomass is an attractive, inexpensive source of potentially fermentable sugars. However, hydrolysis of lignocellulose results in a complex mixture containing microbial inhibitors at variable co...
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Article
Enhancement of NAD(H) pool for formation of oxidized biochemicals in Escherichia coli
The NAD+/NADH ratio and the total NAD(H) play important roles for whole-cell biochemical redox transformations. After the carbon source is exhausted, the degradation of NAD(H) could contribute to a decline in the...
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Article
Synthesis of citramalic acid from glycerol by metabolically engineered Escherichia coli
Citramalic acid (citramalate) serves as a five-carbon precursor for the chemical synthesis of methacrylic acid. We compared citramalate and acetate accumulation from glycerol using Escherichia coli strains expres...
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Article
Quercetin Glucoside Production by Engineered Escherichia coli
Escherichia coli strains expressing the O-glucosyltransferases UGT73B3 or UGT84B1 were compared for the production of glucosides from quercetin supplied into a defined medium. The formati...
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Article
Open AccessEliminating acetate formation improves citramalate production by metabolically engineered Escherichia coli
Citramalate, a chemical precursor to the industrially important methacrylic acid (MAA), can be synthesized using Escherichia coli overexpressing citramalate synthase (cimA gene). Deletion of gltA encoding citrate...
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Article
Erratum to: Transcriptional analysis and adaptive evolution of Escherichia coli strains growing on acetate
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Article
Transcriptional analysis and adaptive evolution of Escherichia coli strains growing on acetate
Eighteen strains of Escherichia coli were compared for maximum specific growth rate (μ MAX) on 85 mM acetate as the sole carbon source. The C strain ATCC8739 had the greatest growth rate (...
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Article
Isolation and Characterization of Bacteria That Use Furans as the Sole Carbon Source
Five bacterial strains were isolated from wastewater treatment facilities which were able to use furfural as the sole carbon source. Based on 16S rRNA phylogenetic analysis, these strains were identified as Cupri...
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Article
Microbial production of lactic acid
Lactic acid is an important commodity chemical having a wide range of applications. Microbial production effectively competes with chemical synthesis methods because biochemical synthesis permits the generatio...
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Article
Production of biomass and filamentous hemagglutinin by Bordetella bronchiseptica
The mammalian pathogen Bordetella bronchiseptica was grown under controlled batch conditions with glutamate as the primary carbon and nitrogen source. First, a Box-Behnken statistical design quantified the effect...
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Article
Differential sensitivities of the growth of Escherichia coli to acrylate under aerobic and anaerobic conditions and its effect on product formation
The effect of acrylate on the growth of Escherichia coli was determined under aerobic and anaerobic conditions in glucose-defined medium. Growth occurred with up to 35 mM acrylate under aerobic conditions but cea...
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Article
Open AccessEffect of overexpressing nhaA and nhaR on sodium tolerance and lactate production in Escherichia coli
Like other bacteria, Escherichia coli must carefully regulate the intracellular concentration of sodium ion (Na+). During the bacterial production of any organic acid, cations like Na+ invariably accumulate durin...
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Article
Continuous-flow ferrohydrodynamic sorting of particles and cells in microfluidic devices
A new sorting scheme based on ferrofluid hydrodynamics (ferrohydrodynamics) was used to separate mixtures of particles and live cells simultaneously. Two species of cells, including Escherichia coli and Saccharom...
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Article
Open AccessSimultaneous utilization of glucose, xylose and arabinose in the presence of acetate by a consortium of Escherichia coli strains
The efficient microbial utilization of lignocellulosic hydrolysates has remained challenging because this material is composed of multiple sugars and also contains growth inhibitors such as acetic acid (acetat...
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Article
Microbial removal of acetate selectively from sugar mixtures
Acetic acid is an unavoidable constituent of the biomass hydrolysates generated from acetylated hemicellulose and lignin, and acetate affects the performance of microbes used to convert these hydrolysates into...
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Article
Effect of flue gas components on succinate production and CO2 fixation by metabolically engineered Escherichia coli
Escherichia coli pfl ldhA ptsG (AFP111) was studied for succinate production in defined medium using trace gases typically found in flue gas; i.e. oxygen (O2), nitrogen dioxide (NO2), sulfur dioxide (SO2) and car...
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Article
Conversion of glycerol to pyruvate by Escherichia coli using acetate- and acetate/glucose-limited fed-batch processes
We report the conversion of glycerol to pyruvate by E. coli ALS929 containing knockouts in the genes encoding for phosphoenolpyruvate synthase, lactate dehydrogenase, pyruvate formate lyase, the pyruvate dehydrog...
