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Protocol
In Silico Gene Selection for Custom Oligonucleotide Microarray Design
A method for systematically selecting the large number of sequences needed to custom design an oligonucleotide microarray was presented. This approach uses a Perl script to query sequence databases with gene l...
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Protocol
The Use of Microarray Technology in Nonmammalian Vertebrate Systems
Among vertebrates, the mammalian systems that are frequently used to investigate questions related to human health have gained the most benefit from microarray technology to date. However, it is clear that bio...
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Article
Open AccessTranscriptome of neonatal preBötzinger complex neurones in Dbx1 reporter mice
We sequenced the transcriptome of brainstem interneurons in the specialized respiratory rhythmogenic site dubbed preBötzinger Complex (preBötC) from newborn mice. To distinguish molecular characteristics of th...
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Protocol
Histological Observation of Teratogenic Phenotypes Induced in Frog Embryo Assays
Amphibian embryos have long served as an ideal model for teratogenicity testing. While whole-mount embryo observations can be utilized, histological observation of teratogenic phenotypes provides a wealth of a...
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Article
Open AccessThe genetic insulator RiboJ increases expression of insulated genes
A primary objective of synthetic biology is the construction of genetic circuits with behaviors that can be predicted based on the properties of the constituent genetic parts from which they are built. However...
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Article
Mercury delays cerebellar development in a model songbird species, the zebra finch
Mercury exposure can disrupt development of the cerebellum, part of the brain essential for coordination of movement through a complex environment, including flight. In precocial birds, such as fowl, the cereb...
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Article
Open AccessTranscriptomes of electrophysiologically recorded Dbx1-derived respiratory neurons of the preBötzinger complex in neonatal mice
Breathing depends on interneurons in the preBötzinger complex (preBötC) derived from Dbx1-expressing precursors. Here we investigate whether rhythm- and pattern-generating functions reside in discrete classes of ...
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Article
Open AccessSingle cell transcriptome sequencing of inspiratory neurons of the preBötzinger complex in neonatal mice
Neurons in the brainstem preBötzinger complex (preBötC) generate the rhythm and rudimentary motor pattern for inspiratory breathing movements. We performed whole-cell patch-clamp recordings from inspiratory ne...
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Article
Open AccessTranscriptional dynamics during Rhodococcus erythropolis infection with phage WC1
Belonging to the Actinobacteria phylum, members of the Rhodococcus genus thrive in soil, water, and even intracellularly. While most species are non-pathogenic, several cause respiratory disease in animals and, m...