Abstract
Amphibian embryos have long served as an ideal model for teratogenicity testing. While whole-mount embryo observations can be utilized, histological observation of teratogenic phenotypes provides a wealth of additional information that can lead to mechanistic insights. In this chapter, detailed protocols for two methods of sectioning embryos as well as a guide for histological analysis is provided.
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Acknowledgments
This work was supported by NIH (1R15HD077624-01 to MSS), NSF (1257895 to MSS), Howard Hughes Medical Institute Science Education Program Grant to the College of William and Mary, and the College of William and Mary Roy R. Charles Center Honors Fellowship to MEP.
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Pownall, M.E., Saha, M.S. (2018). Histological Observation of Teratogenic Phenotypes Induced in Frog Embryo Assays. In: Félix, L. (eds) Teratogenicity Testing. Methods in Molecular Biology, vol 1797. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7883-0_16
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DOI: https://doi.org/10.1007/978-1-4939-7883-0_16
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