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Article
Open AccessComparative analysis of novel and common reference genes in adult tissues of the mussel Mytilus galloprovincialis
Real-time quantitative PCR is a widely used method for gene expression analyses in various organisms. Its accuracy mainly relies on the correct selection of reference genes. Any experimental plan involving rea...
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Article
Author Correction: The mitochondrial protein Opa1 promotes adipocyte browning that is dependent on urea cycle metabolites
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Protocol
Detecting Structural Variants and Associated Gene Presence–Absence Variation Phenomena in the Genomes of Marine Organisms
As complete genomes become easier to attain, even from previously difficult-to-sequence species, and as genomic resequencing becomes more routine, it is becoming obvious that genomic structural variation is mo...
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Article
The mitochondrial protein Opa1 promotes adipocyte browning that is dependent on urea cycle metabolites
White to brown/beige adipocytes conversion is a possible therapeutic strategy to tackle the current obesity epidemics. While mitochondria are key for energy dissipation in brown fat, it is unknown if they can ...
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Article
Open AccessInvestigation of the activity of transposable elements and genes involved in their silencing in the newt Cynops orientalis, a species with a giant genome
Caudata is an order of amphibians with great variation in genome size, which can reach enormous dimensions in salamanders. In this work, we analysed the activity of transposable elements (TEs) in the transcrip...
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Article
Open AccessMassive gene presence-absence variation shapes an open pan-genome in the Mediterranean mussel
The Mediterranean mussel Mytilus galloprovincialis is an ecologically and economically relevant edible marine bivalve, highly invasive and resilient to biotic and abiotic stressors causing recurrent massive morta...
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Article
Open AccessThe transcriptome of the newt Cynops orientalis provides new insights into evolution and function of sexual gene networks in sarcopterygians
Amphibians evolved in the Devonian period about 400 Mya and represent a transition step in tetrapod evolution. Among amphibians, high-throughput sequencing data are very limited for Caudata, due to their large...
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Protocol
Purification and Functional Characterization of the Effects on Cell Signaling of Mytilectin: A Novel β-Trefoil Lectin from Marine Mussels
In the 2010s, a novel lectin family with β-trefoil folding has been identified in marine mussels from the family Mytilidae (phylum Mollusca). “MytiLec-1,” the lectin described in this chapter, was the first me...
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Article
Open AccessStructural determinants of CO2-sensitivity in the β connexin family suggested by evolutionary analysis
A subclade of connexins comprising Cx26, Cx30, and Cx32 are directly sensitive to CO2. CO2 binds to a carbamylation motif present in these connexins and causes their hemichannels to open. Cx26 may contribute to C...
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Article
Open AccessMolecular and Structural Characterization of MHC Class II β Genes Reveals High Diversity in the Cold-Adapted Icefish Chionodraco hamatus
This study reports the presence of two distinct MHC class II β genes in the Antarctic icefish Chionodraco hamatus, belonging to the classical (ChhaDAB) and nonclassical (ChhaDBB) evolutionary lineages. By the app...
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Article
Open AccessDietary fatty acid source has little effect on the development of the immune system in the pyloric caeca of Atlantic salmon fry
The quality and relative amounts of dietary lipids may affect the health and growth of cultured Atlantic salmon. So far, little is known about their effects on the performance of the fish immune system during ...
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Chapter
Biotechnologies from Marine Bivalves
Bivalve molluscs comprise more than 9000 extant species. A number of them are traditionally farmed worldwide and are fundamental in the functioning of benthic ecosystems. The peculiarities of marine bivalves h...
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Article
Open AccessParallel identification of novel antimicrobial peptide sequences from multiple anuran species by targeted DNA sequencing
Antimicrobial peptides (AMPs) are multifunctional effector molecules that often combine direct antimicrobial activities with signaling or immunomodulatory functions. The skin secretions of anurans contain a va...
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Chapter
Immunity in Molluscs: Recognition and Effector Mechanisms, with a Focus on Bivalvia
The study of molluscan immune systems, in particular those of bivalve molluscs (e.g., clams, oysters, scallops, mussels), has experienced great growth in recent decades, mainly due to the needs of a rapidly gr...
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Article
Open AccessThe purplish bifurcate mussel Mytilisepta virgata gene expression atlas reveals a remarkable tissue functional specialization
Mytilisepta virgata is a marine mussel commonly found along the coasts of Japan. Although this species has been the subject of occasional studies concerning its ecological role, growth...
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Article
Open AccessEvolution of Th2 responses: characterization of IL-4/13 in sea bass (Dicentrarchus labrax L.) and studies of expression and biological activity
Th2 immunity is a primary host defence against metazoan pathogens and two of the important cytokines involved in this immune response in mammals are IL-4 and IL-13. Recently the origin and evolution of Th2 imm...
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Article
Open AccessImmunoglobulin T from sea bass (Dicentrarchus labrax L.): molecular characterization, tissue localization and expression after nodavirus infection
Immunoglobulins (Igs) are fundamental components of the adaptive immune system of vertebrates, with the IgT/IgZ isotype specific of Teleosts. In this paper we describe the identification of an IgT heavy chain ...
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Article
New features of desiccation tolerance in the lichen photobiont Trebouxia gelatinosa are revealed by a transcriptomic approach
Trebouxia is the most common lichen-forming genus of aero-terrestrial green algae and all its species are desiccation tolerant (DT). The molecular bases of this remarkable adaptation are,...
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Article
Open AccessThe Lungfish Transcriptome: A Glimpse into Molecular Evolution Events at the Transition from Water to Land
Lungfish and coelacanths are the only living sarcopterygian fish. The phylogenetic relationship of lungfish to the last common ancestor of tetrapods and their close morphological similarity to their fossil anc...
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Article
Open AccessRNA sequencing and de novo assembly of the digestive gland transcriptome in Mytilus galloprovincialis fed with toxinogenic and non-toxic strains of Alexandrium minutum
The Mediterranean mussel Mytilus galloprovincialis is marine bivalve with a relevant commercial importance as well as a key sentinel organism for the biomonitoring of environmental pollution. Here we report the R...
