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Open AccessCryopreservation of cerebrospinal fluid cells preserves the transcriptional landscape for single-cell analysis
Cerebrospinal fluid (CSF) matrix biomarkers have become increasingly valuable surrogate markers of neuropsychiatric diseases in research and clinical practice. In contrast, CSF cells have been rarely investiga...
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Article
Open AccessEffect of transcription inhibition and generation of suppressive viral non-coding RNAs
HIV-1 patients receiving combination antiretroviral therapy (cART) survive infection but require life-long adherence at high expense. In chronic cART-treated patients with undetectable viral titers, cell-assoc...
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Article
The impact of skeletal muscle on the pharmacokinetics and toxicity of 5-fluorouracil in colorectal cancer
Great heterogeneity exists in the ability of adults with cancer to tolerate chemotherapy. Variability in body composition may affect rates of metabolism of cytotoxic agents and contribute to the variable chemo...
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Article
Open AccessssAAVs containing cassettes encoding SaCas9 and guides targeting hepatitis B virus inactivate replication of the virus in cultured cells
Management of infection with hepatitis B virus (HBV) remains a global health problem. Persistence of stable covalently closed circular DNA (cccDNA) during HBV replication is responsible for modest curative eff...
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Article
The Application of CRISPR/Cas9 Technologies and Therapies in Stem Cells
The ability to precisely modify genomic sequences has always been a powerful tool for determining the relationship between genotype and phenotype, and for targeted editing of disease-causing genetic lesions. U...
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Protocol
Synthetic SiRNA Delivery: Progress and Prospects
Small interfering RNA (siRNA) is a powerful tool for modulating gene expression by RNA interference (RNAi). Duplex RNA oligonucleotides induce cleavage of homologous target transcripts, thereby enabling posttr...
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Article
Quantification of nascent transcription by bromouridine immunocapture nuclear run-on RT-qPCR
Roberts et al. describe how to measure transcriptional activity using nuclear run-on RT-qPCR assays. The approach is ideal for analyzing a single gene (or small number of genes) or for follow-up or validation of ...
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Protocol
Construction of Mismatched Inverted Repeat (IR) Silencing Vectors for Maximizing IR Stability and Effective Gene Silencing in Plants
Inverted repeat (IR) RNA silencing vectors containing homologous fragments of target endogenous plant genes, or pathogen genes, are the most widely used vectors to either study the function of genes involved i...
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Article
Open AccessThe 37kDa/67kDa Laminin Receptor acts as a receptor for Aβ42 internalization
Neuronal loss is a major neuropathological hallmark of Alzheimer's disease (AD). The associations between soluble Aβ oligomers and cellular components cause this neurotoxicity. The 37 kDa/67 kDa laminin recept...
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Open AccessAnti-LRP/LR specific antibody IgG1-iS18 and knock-down of LRP/LR by shRNAs rescue cells from Aβ42 induced cytotoxicity
Alzheimer's disease (AD) is characterized by neurofibrillary tangles, senile plaques and neuronal loss. Amyloid beta (Aβ) is proposed to elicit neuronal loss through cell surface receptors. As Aβ shares common...
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Open AccessAnti-LRP/LR specific antibodies and shRNAs impede amyloid beta shedding in Alzheimer's disease
Alzheimer's disease (AD) is the most prevalent form of dementia. The amyloid beta (Aβ) peptide is the predominant candidate aetiological agent and is generated through the sequential proteolytic cleavage of th...
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Open AccessImpact of sustained RNAi-mediated suppression of cellular cofactor Tat-SF1 on HIV-1 replication in CD4+ T cells
Conventional anti-HIV drug regimens targeting viral enzymes are plagued by the emergence of drug resistance. There is interest in targeting HIV-dependency factors (HDFs), host proteins that the virus requires ...
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Article
Progress in the use of RNA interference as a therapy for chronic hepatitis B virus infection
Chronic infection with hepatitis B virus (HBV) occurs in approximately 6% of the world's population and carriers of the virus are at risk for hepatocellular carcinoma and cirrhosis. Current treatment regimens,...
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Protocol
Effective Pol III-Expressed Long Hairpin RNAs Targeted to Multiple Unique Sites of HIV-1
The RNA interference (RNAi) pathway has in recent years been exploited for the development of novel antiviral therapies. The emergence of viral escape mutants, however, is a major impediment to the use of RNAi...
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Article
Defect-Induced Shifts in the Elastic Constants of Silicon
As MEMS devices become ever more sensitive, even slight shifts in materials properties can be detrimental to device performance. Radiation-induced defects can change both the dimensions and mechanical properti...
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Urinary Kininogen: A Possible Regulator of Kinin Formation in Normal Individuals and Subjects with Essential Hypertension, End-Stage Renal and Liver Disease
Most previous studies have not significantly correlated urinary kallikrein to urinary kinins. We investigated whether urinary kininogen might influence kinin formation within the urine. On an ad-lib diet the 2...
