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Article
Open AccessMegahertz pulse trains enable multi-hit serial femtosecond crystallography experiments at X-ray free electron lasers
The European X-ray Free Electron Laser (XFEL) and Linac Coherent Light Source (LCLS) II are extremely intense sources of X-rays capable of generating Serial Femtosecond Crystallography (SFX) data at megahertz ...
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Article
Open AccessSegmented flow generator for serial crystallography at the European X-ray free electron laser
Serial femtosecond crystallography (SFX) with X-ray free electron lasers (XFELs) allows structure determination of membrane proteins and time-resolved crystallography. Common liquid sample delivery continuousl...
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Article
Open AccessMegahertz single-particle imaging at the European XFEL
The emergence of high repetition-rate X-ray free-electron lasers (XFELs) powered by superconducting accelerator technology enables the measurement of significantly more experimental data per day than was previ...
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Article
Open AccessAuthor Correction: Membrane protein megahertz crystallography at the European XFEL
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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Article
Open AccessMembrane protein megahertz crystallography at the European XFEL
The world’s first superconducting megahertz repetition rate hard X-ray free-electron laser (XFEL), the European XFEL, began operation in 2017, featuring a unique pulse train structure with 886 ns between pulse...
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Article
Open AccessAuthor Correction: Coherent diffractive imaging of microtubules using an X-ray laser
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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Article
Open AccessCoherent diffractive imaging of microtubules using an X-ray laser
X-ray free electron lasers (XFELs) create new possibilities for structural studies of biological objects that extend beyond what is possible with synchrotron radiation. Serial femtosecond crystallography has a...
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Article
Open AccessMHz data collection of a microcrystalline mixture of different jack bean proteins
We provide a detailed description of a serial femtosecond crystallography (SFX) dataset collected at the European X-ray free-electron laser facility (EuXFEL). The EuXFEL is the first high repetition rate XFEL ...
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Article
Open AccessMegahertz serial crystallography
The new European X-ray Free-Electron Laser is the first X-ray free-electron laser capable of delivering X-ray pulses with a megahertz inter-pulse spacing, more than four orders of magnitude higher than previou...
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Article
Open AccessMegahertz data collection from protein microcrystals at an X-ray free-electron laser
X-ray free-electron lasers (XFELs) enable novel experiments because of their high peak brilliance and femtosecond pulse duration. However, non-superconducting XFELs offer repetition rates of only 10–120 Hz, pl...
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Article
Open AccessX-ray focusing with efficient high-NA multilayer Laue lenses
Multilayer Laue lenses are volume diffraction elements for the efficient focusing of X-rays. With a new manufacturing technique that we introduced, it is possible to fabricate lenses of sufficiently high numer...
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Article
Open AccessCorrection: Corrigendum: Diffraction data of core-shell nanoparticles from an X-ray free electron laser
Scientific Data 4:170048 doi: 10.1038/sdata201748 (2017); Published 11 April 2017; Updated 24 October 2017. The Data Descriptor incorrectly states the number of normal incidences used to generate the plot in F...
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Article
Open AccessDiffraction data of core-shell nanoparticles from an X-ray free electron laser
X-ray free-electron lasers provide novel opportunities to conduct single particle analysis on nanoscale particles. Coherent diffractive imaging experiments were performed at the Linac Coherent Light Source (LC...
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Article
De novo phasing with X-ray laser reveals mosquito larvicide BinAB structure
BinAB is a naturally occurring paracrystalline larvicide distributed worldwide to combat the devastating diseases borne by mosquitoes. These crystals are composed of homologous molecules, BinA and BinB, which ...
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Article
Liquid explosions induced by X-ray laser pulses
Explosions are spectacular and intriguing phenomena that expose the dynamics of matter under extreme conditions. We investigated, using time-resolved imaging, explosions induced by ultraintense X-ray laser pul...
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Article
Open AccessEstablishing nonlinearity thresholds with ultraintense X-ray pulses
X-ray techniques have evolved over decades to become highly refined tools for a broad range of investigations. Importantly, these approaches rely on X-ray measurements that depend linearly on the number of inc...
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Article
Open AccessSingle-shot diffraction data from the Mimivirus particle using an X-ray free-electron laser
Free-electron lasers (FEL) hold the potential to revolutionize structural biology by producing X-ray pules short enough to outrun radiation damage, thus allowing imaging of biological samples without the limit...
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Article
Open AccessSerial femtosecond crystallography datasets from G protein-coupled receptors
We describe the deposition of four datasets consisting of X-ray diffraction images acquired using serial femtosecond crystallography experiments on microcrystals of human G protein-coupled receptors, grown and...
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Article
Femtosecond and nanometre visualization of structural dynamics in superheated nanoparticles
Single Xe clusters are superheated using an intense optical laser pulse and the structural evolution is imaged with a single X-ray pulse. Ultrafast surface softening on the nanometre scale is resolved within 1...
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Article
Open AccessTernary structure reveals mechanism of a membrane diacylglycerol kinase
Diacylglycerol kinase catalyses the ATP-dependent conversion of diacylglycerol to phosphatidic acid in the plasma membrane of Escherichia coli. The small size of this integral membrane trimer, which has 121 resid...
