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Article
Open AccessObservation of a single protein by ultrafast X-ray diffraction
The idea of using ultrashort X-ray pulses to obtain images of single proteins frozen in time has fascinated and inspired many. It was one of the arguments for building X-ray free-electron lasers. According to ...
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Article
Open AccessMegahertz single-particle imaging at the European XFEL
The emergence of high repetition-rate X-ray free-electron lasers (XFELs) powered by superconducting accelerator technology enables the measurement of significantly more experimental data per day than was previ...
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Article
Femtosecond X-ray Fourier holography imaging of free-flying nanoparticles
Ultrafast X-ray imaging on individual fragile specimens such as aerosols 1 , metastable particles 2 , superfluid quantum systems ...
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Article
Open AccessCoherent soft X-ray diffraction imaging of coliphage PR772 at the Linac coherent light source
Single-particle diffraction from X-ray Free Electron Lasers offers the potential for molecular structure determination without the need for crystallization. In an effort to further develop the technique, we pr...
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Article
Open AccessA data set from flash X-ray imaging of carboxysomes
Ultra-intense femtosecond X-ray pulses from X-ray lasers permit structural studies on single particles and biomolecules without crystals. We present a large data set on inherently heterogeneous, polyhedral car...
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Article
Open AccessSingle-shot diffraction data from the Mimivirus particle using an X-ray free-electron laser
Free-electron lasers (FEL) hold the potential to revolutionize structural biology by producing X-ray pules short enough to outrun radiation damage, thus allowing imaging of biological samples without the limit...
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Open AccessOpen data set of live cyanobacterial cells imaged using an X-ray laser
Structural studies on living cells by conventional methods are limited to low resolution because radiation damage kills cells long before the necessary dose for high resolution can be delivered. X-ray free-ele...
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Article
Imaging single cells in a beam of live cyanobacteria with an X-ray laser
There exists a conspicuous gap of knowledge about the organization of life at mesoscopic levels. Ultra-fast coherent diffractive imaging with X-ray free-electron lasers can probe structures at the relevant len...
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Article
High-throughput imaging of heterogeneous cell organelles with an X-ray laser
We overcome two of the most daunting challenges in single-particle diffractive imaging: collecting many high-quality diffraction patterns on a small amount of sample and separating components from mixed sample...
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Article
Lipidic phase membrane protein serial femtosecond crystallography
Lipidic sponge phase crystallization yields membrane protein microcrystals that can be injected into an X-ray free electron laser beam, yielding diffraction patterns that can be processed to recover the crysta...
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Article
In vivo protein crystallization opens new routes in structural biology
Expression of a protein in Sf9 insect cells at high concentration triggers formation of in vivo crystals that can be analyzed by serial femtosecond X-ray crystallography.
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Article
Self-terminating diffraction gates femtosecond X-ray nanocrystallography measurements
X-ray free-electron lasers have enabled new approaches to the structural determination of protein crystals that are too small or radiation-sensitive for conventional analysis1. For sufficiently short pulses, diff...
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Article
Single mimivirus particles intercepted and imaged with an X-ray laser
The start-up of the Linac Coherent Light Source (LCLS), the new femtosecond hard X-ray laser facility in Stanford, California, has brought high expectations of a new era for biological imaging. The intense, ul...
