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Protocol
Particle Exclusion Assay: A Tool for Measuring Hyaluronan Pericellular Matrix
Hyaluronan (HA) is the most abundant glycosaminoglycan in the extracellular , and its deposition is strictly related to changes in cellular behaviors, such as , proliferation, and adhesion. Pericellular HA i...
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Chapter
Biochemistry of Hyaluronan Synthesis
Hyaluronan (HA) is a ubiquitous component of the extracellular matrix and cell microenvironment made of β D-Glucuronic acid and D-N-Acetylglucosaminedisaccharides bound by alternating glycosidic β(1,3) and β(1...
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Chapter
Long Noncoding RNAs and Epigenetic Regulation of Hyaluronan Synthesis
Hyaluronan (HA) is a ubiquitous glycosaminglycan of exracellular matrix, whose synthesis is due to the activity of three transmembrane isoenzymes named hyaluronan synthases 1, 2, and 3 (HAS1–3). The regulation...
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Protocol
A Nonradioactive Method to Measure Hyaluronan Synthase Activity
Hyaluronan (HA) is a component of the extracellular matrix that is involved in many physiological and pathological processes. As HA modulates several functions (i.e., cell proliferation and migration, inflammatio...
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Article
Open AccessInvolvement of hyaluronan in the adaptive changes of the rat small intestine neuromuscular function after ischemia/reperfusion injury
Intestinal ischemia/reperfusion (I/R) injury has severe consequences on myenteric neurons, which can be irreversibly compromised resulting in slowing of transit and hindered food digestion. Myenteric neurons s...
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Chapter
Heparan Sulfate in the Tumor Microenvironment
The biology of tumor cells strictly depends on their microenvironment architecture and composition, which controls the availability of growth factors and signaling molecules. Thus, the network of glycosaminogl...
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Protocol
Method for Studying ECM Expression: In Situ RT-PCR
The gene expression of the extracellular matrix macromolecules is critical in the analysis of various pathologies. The use of a RT-PCR directly on a fixed tissue enables the recognition of the real expressing ...
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Article
Open AccessChanges in hyaluronan deposition in the rat myenteric plexus after experimentally-induced colitis
Myenteric plexus alterations hamper gastrointestinal motor function during intestinal inflammation. Hyaluronan (HA), an extracellular matrix glycosaminoglycan involved in inflammatory responses, may play a rol...
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Article
MDA-MB-231 breast cancer cell viability, motility and matrix adhesion are regulated by a complex interplay of heparan sulfate, chondroitin−/dermatan sulfate and hyaluronan biosynthesis
Proteoglycans and glycosaminoglycans modulate numerous cellular processes relevant to tumour progression, including cell proliferation, cell-matrix interactions, cell motility and invasive growth. Among the gl...
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Article
Biology and biotechnology of hyaluronan
The hyaluronan (HA) polymer is a critical component of extracellular matrix with a remarkable structure: is a linear and unbranched polymer without sulphate or phosphate groups. It is ubiquitous in mammals sho...
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Protocol
Fast Screening of Glycosaminoglycan Disaccharides by Fluorophore-Assisted Carbohydrate Electrophoresis (FACE): Applications to Biologic Samples and Pharmaceutical Formulations
Hyaluronan (HA), chondroitin sulfate (CS), and heparan sulfate (HS) are glycosaminoglycans (GAGs) with a great importance in biological processes as they participate in functional cell properties, such as migr...
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Protocol
Analysis of Hyaluronan Synthase Activity
Hyaluronan (HA) is a component of the extracellular matrix that is involved in many physiological and pathological processes. As HA modulates several functions (i.e., cell proliferation and migration, inflamma...
