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Article
Host–microbe metabolic dialogue
Metabolomics and feeding experiments demonstrate the host’s active role in sharing organic acids with a gut microbiota member, revealing host–microbe interactions that foster symbiosis.
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Article
Open AccessmicrobeMASST: a taxonomically informed mass spectrometry search tool for microbial metabolomics data
microbeMASST, a taxonomically informed mass spectrometry (MS) search tool, tackles limited microbial metabolite annotation in untargeted metabolomics experiments. Leveraging a curated database of >60,000 micro...
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Article
Visualization of metabolites and microbes at high spatial resolution using MALDI mass spectrometry imaging and in situ fluorescence labeling
Label-free molecular imaging techniques such as matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI-MSI) enable the direct and simultaneous map** of hundreds of different metabolites...
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Article
Open AccessSugars dominate the seagrass rhizosphere
Seagrasses are among the most efficient sinks of carbon dioxide on Earth. While carbon sequestration in terrestrial plants is linked to the microorganisms living in their soils, the interactions of seagrasses ...
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Article
Open AccessDifferential regulation of degradation and immune pathways underlies adaptation of the ectosymbiotic nematode Laxus oneistus to oxic-anoxic interfaces
Eukaryotes may experience oxygen deprivation under both physiological and pathological conditions. Because oxygen shortage leads to a reduction in cellular energy production, all eukaryotes studied so far cons...
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Article
Open AccessDie chemische Sprache von Symbiosen sichtbar machen
Small molecules — metabolites — provide the basis for chemical interactions between hosts and microbes. Especially in animal-microbe symbioses, the close physical interactions require a spatial organization of...
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Article
Verrucomicrobia use hundreds of enzymes to digest the algal polysaccharide fucoidan
Brown algae are important players in the global carbon cycle by fixing carbon dioxide into 1 Gt of biomass annually, yet the fate of fucoidan—their major cell wall polysaccharide—remains poorly understood. Mic...
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Article
Spatial metabolomics of in situ host–microbe interactions at the micrometre scale
Spatial metabolomics describes the location and chemistry of small molecules involved in metabolic phenotypes, defence molecules and chemical interactions in natural communities. Most current techniques are un...
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Article
Open AccessTwo intracellular and cell type-specific bacterial symbionts in the placozoan Trichoplax H2
Placozoa is an enigmatic phylum of simple, microscopic, marine metazoans1,2. Although intracellular bacteria have been found in all members of this phylum, almost nothing is known about their identity, location a...
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Article
Open AccessUnique metabolites protect earthworms against plant polyphenols
All higher plants produce polyphenols, for defence against above-ground herbivory. These polyphenols also influence the soil micro- and macro-fauna that break down plant leaf litter. Polyphenols therefore indi...
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Article
Bayesian deconvolution and quantification of metabolites in complex 1D NMR spectra using BATMAN
BATMAN is an R package developed to analyze one-dimensional NMR spectra of complex metabolite mixtures using a user-defined template library.
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Article
Open AccessRole of N-terminal protein formylation in central metabolic processes in Staphylococcus aureus
Bacterial protein biosynthesis usually depends on a formylated methionyl start tRNA but Staphylococcus aureus is viable in the absence of Fmt, the tRNAMet formyl transferase. fmt mutants exhibit reduced growth ra...
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Article
Tissue disruption and extraction methods for metabolic profiling of an invertebrate sentinel species
Metabolic profiling of tissues needs special attention, because the compartmentalization of cellular constituents will be abolished by sample homogenization. This loss of partitioning leads to protein and meta...
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Protocol
Metabolome Analysis of Gram-Positive Bacteria such as Staphylococcus aureus by GC-MS and LC-MS
The field of metabolomics has become increasingly important in the context of functional genomics. Together with other ”omics“ data, the investigation of the metabolome is an essential part of systems biology....
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Article
Open AccessFed-batch process for the psychrotolerant marine bacterium Pseudoalteromonas haloplanktis
Pseudoalteromonas haloplanktis is a cold-adapted γ-proteobacterium isolated from Antarctic sea ice. It is characterized by remarkably high growth rates at low temperatures. P. haloplanktis is one of the model org...
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Article
Regulation of acetoin and 2,3-butanediol utilization in Bacillus licheniformis
The acoABCL and acuABC operons of Bacillus licheniformis DSM13 are strongly induced at the transcriptional level during glucose starvation conditions. Primer extension analyses of this study indicate that the aco...
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Article
Chemical characterization of soil extract as growth media for the ecophysiological study of bacteria
We investigated the composition of soil-extracted solubilized organic and inorganic matter (SESOM) prepared from three different soils. Growth of various bacterial strains in these soil extracts was evaluated ...
