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Article
Open AccessAnalysis and imaging of biocidal agrochemicals using ToF-SIMS
ToF-SIMS has been increasingly widely used in recent years to look at biological matrices, in particular for biomedical research, although there is still a lot of development needed to maximise the value of th...
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Article
Open AccessRapid screening of cellular stress responses in recombinant Pichia pastoris strains using metabolite profiling
Heterologous protein production in the yeast Pichia pastoris can be limited by biological responses to high expression levels; the unfolded protein response (UPR) is a key determinant of the success of protein pr...
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Article
Open AccessModelling the acid/base 1H NMR chemical shift limits of metabolites in human urine
Despite the use of buffering agents the 1H NMR spectra of biofluid samples in metabolic profiling investigations typically suffer from extensive peak frequency shifting between spectra. These chemical shift chang...
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Article
Open AccessUnique metabolites protect earthworms against plant polyphenols
All higher plants produce polyphenols, for defence against above-ground herbivory. These polyphenols also influence the soil micro- and macro-fauna that break down plant leaf litter. Polyphenols therefore indi...
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Article
Bayesian deconvolution and quantification of metabolites in complex 1D NMR spectra using BATMAN
BATMAN is an R package developed to analyze one-dimensional NMR spectra of complex metabolite mixtures using a user-defined template library.
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Protocol
Metabolic Footprinting: Extracellular Metabolomic Analysis
Uptake and excretion of nutrients is an integral part of a cell’s physiology. Using analytical chemistry techniques, metabolite uptake and excretion from the culture medium can be quantified. As cellular metab...
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Protocol
Profiling the Metabolic Signature of Senescence
Aging is a complex process, which involves changes in different cellular functions that all can be integrated on the metabolite level. This means that different gene regulation pathways that affect aging might...
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Article
Tissue disruption and extraction methods for metabolic profiling of an invertebrate sentinel species
Metabolic profiling of tissues needs special attention, because the compartmentalization of cellular constituents will be abolished by sample homogenization. This loss of partitioning leads to protein and meta...
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Article
Open AccessMetabolic profiling detects early effects of environmental and lifestyle exposure to cadmium in a human population
The 'exposome' represents the accumulation of all environmental exposures across a lifetime. Top-down strategies are required to assess something this comprehensive, and could transform our understanding of ho...
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Article
Open AccessA metabolic signature of long life in Caenorhabditis elegans
Many Caenorhabditis elegans mutations increase longevity and much evidence suggests that they do so at least partly via changes in metabolism. However, up until now there has been no systematic investigation of h...
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Article
Validation of metabolomics for toxic mechanism of action screening with the earthworm Lumbricus rubellus
One of the promises of environmental metabolomics, together with other ecotoxicogenomic approaches, is that it can give information on toxic compound mechanism of action (MOA), by providing a specific response...
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Article
Environmental metabolomics: a critical review and future perspectives
Environmental metabolomics is the application of metabolomics to characterise the interactions of organisms with their environment. This approach has many advantages for studying organism–environment interacti...
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Article
Open Access'Systems toxicology' approach identifies coordinated metabolic responses to copper in a terrestrial non-model invertebrate, the earthworm Lumbricus rubellus
New methods are needed for research into non-model organisms, to monitor the effects of toxic disruption at both the molecular and functional organism level. We exposed earthworms (Lumbricus rubellus Hoffmeister)...
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Article
Standard reporting requirements for biological samples in metabolomics experiments: environmental context
Metabolomic technologies are increasingly being applied to study biological questions in a range of different settings from clinical through to environmental. As with other high-throughput technologies, such a...
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Article
NMR-derived developmental metabolic trajectories: an approach for visualizing the toxic actions of trichloroethylene during embryogenesis
Fish embryo toxicity tests for chemical risk assessment have traditionally been based upon non-specific endpoints including morphological abnormalities, hatching success, and mortality. Here we extend the appl...
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Article
Environmental Metabonomics: Applying Combination Biomarker Analysis in Earthworms at a Metal Contaminated Site
Earthworms were taken across an environmental gradient of metal contamination for ecotoxicology assessment. Both indigenous (Lumbricus rubellusand L. terrestris) and introduced earthworms (Eisenia andrei, exposed...
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Article
Investigating the specificity of regulators of degradation of hydrocarbons and hydrocarbon-based compounds using structure-activity relationships
Microbial biosensors which have genes forbioluminescence coupled to genes that controlhydrocarbon degradation pathways can be used asreporters on the specificity of regulation of thosepathways. Structure-activ...
