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Article
jYCaMP: an optimized calcium indicator for two-photon imaging at fiber laser wavelengths
Femtosecond lasers at fixed wavelengths above 1,000 nm are powerful, stable and inexpensive, making them promising sources for two-photon microscopy. Biosensors optimized for these wavelengths are needed for b...
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Article
Labeling cellular structures in vivo using confined primed conversion of photoconvertible fluorescent proteins
Primed conversion of photoconvertible fluorescent proteins enables targeted labeling in vivo with high axial resolution. This Protocol describes how to modify a confocal microscope to perform confined primed conv...