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Article
A Bifunctional-Blocker-Aided Hybridization Chain Reaction Lighting-Up Self-calibrating Nanocluster Fluorescence for Reliable Nucleic Acid Detection
In this work, we proposed a ratiometric silver nanoclusters (AgNCs) fluorescent assay by designing a bifunctional-blocker-aided hybridization chain reaction (HCR). Hairpin probe 1 (HP1) containing two special ...
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Article
On-site preparation of sandwich plasmonic coupled SERS tape toward pesticide residue determination on food surface
A sandwich plasmonic coupled surface enhanced Raman spectroscopy (SERS) tape is proposed prepared by peeling the chemical printed silver nanocorals (AgNCs) from Cu sheet with adhesive tape, which can sample ta...
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Article
Gold nanoparticle dimer–based immunochromatography for in situ ultrasensitive detection of porcine epidemic diarrhea virus
The low detection sensitivity of lateral-flow immunochromatography assay (LFIA) based on spherical gold nanoparticle (AuNP) limits its wide applications. In the present study, AuNP dimers with strong plasma sc...
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Article
Open AccessThe genomic characteristics of RET fusion positive tumors in Chinese non-small cell lung cancer (NSCLC) patients
Approximately 1–2% of non-small cell lung cancer (NSCLC) patients harbor RET (rearranged during transfection) fusions. The oncogenic RET fusions could lead to constitutive kinase activation and oncogenesis.
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Article
A novel fluorescence aptasensor based on PCN-223 as an efficient quencher for sensitive determination of prostate-specific antigen
A novel fluorescence aptasensor based on PCN-223 as an efficient quencher was developed to sensitively detect prostate-specific antigen (PSA). The 5-carboxytetramethylrhodamine (TAMRA)-labeled PSA aptamer was ...
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Article
Open AccessIsland-specific evolution of a sex-primed autosome in a sexual planarian
The sexual strain of the planarian Schmidtea mediterranea, indigenous to Tunisia and several Mediterranean islands, is a hermaphrodite1,2. Here we isolate individual chromosomes and use sequencing, Hi-C3,4 and li...
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Article
Open AccessGenomic epidemiology of the Los Angeles COVID-19 outbreak and the early history of the B.1.43 strain in the USA
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused global disruption of human health and activity. Being able to trace the early outbreak of SARS-CoV-2 within a locality can inform pub...
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Article
Homogeneous photoelectrochemical biosensor for microRNA based on target-responsive hydrogel coupled with exonuclease III and nicking endonuclease Nb.BbvCI assistant cascaded amplification strategy
MicroRNAs can serve as biomarkers for many cancers, so it is significant to develop simple and sensitive strategies for microRNAs detection. Photoelectrochemical (PEC) detection has the advantages of simple eq...
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Article
Massively scaled-up testing for SARS-CoV-2 RNA via next-generation sequencing of pooled and barcoded nasal and saliva samples
Frequent and widespread testing of members of the population who are asymptomatic for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is essential for the mitigation of the transmission of the vir...
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Article
A Bright Nitrogen-doped-Carbon-Dots based Fluorescent Biosensor for Selective Detection of Copper Ions
In this work, a novel fluorescent biosensor has been constructed for rapid detection of Cu(II) via the interaction between the fluorophore groups on the surface of nitrogen-doped-carbon-dots (N-CDs) and ·OH pr...
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Article
Dark field microscope-based single nanoparticle identification coupled with statistical analysis for ultrasensitive biotoxin detection in complex sample matrix
A novel approach for ultrasensitive ochratoxin A (OTA) detection is reported based on dark field microscope-based single nanoparticle identification coupled with a statistical analysis method. OTA aptamers wer...
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Article
Electrochemiluminescence Sensor for Cancer Cell Detection Based on H2O2-Triggered Stimulus Response System
A H2O2-triggered stimulus response electrochemiluminescence (ECL) sensor for sensitive detection of cancer cells using mesoporous silica nanoparticles (MSNs) has been proposed. ECL signal-generating molecules (Ru...
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Article
A fluorescence signal amplification strategy for modification-free ratiometric determination of tyrosinase in situ based on the use of dual-templated copper nanoclusters
A fluorescence resonance energy transfer (FRET)-based in situ fluorescence signal amplification strategy is described for the determination of tyrosinase (TYR). In this assay, a dual-templated copper nanoclust...
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Article
Open AccessIntratumoral heterogeneity of EGFR-activating mutations in advanced NSCLC patients at the single-cell level
Intratumoral epidermal growth factor receptor (EGFR) mutational heterogeneity is yet controversial in non-small cell lung cancer (NSCLC) patients. Single-cell analysis provides the genetic profile of single cance...
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Article
Rapid synthesis of a highly active and uniform 3-dimensional SERS substrate for on-spot sensing of dopamine
A rapid method is described for the preparation of a highly uniform and sensitive SERS substrate by an improved ‘drop-and-dry’ method. Gold nanobipyramids (Au NBPs) were prepared inside the nanoholes (nanowall...
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Article
Fluorometric determination of the activity of inorganic pyrophosphatase and its inhibitors by exploiting the peroxidase mimicking properties of a two-dimensional metal organic framework
A copper(II)-based two-dimensional metal-organic framework with nanosheet structure (CuBDC NS) that possesses peroxidase (POx) mimicking activity was prepared. In the presence of hydrogen peroxide, the system ...
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Article
A calcium alginate sponge with embedded gold nanoparticles as a flexible SERS substrate for direct analysis of pollutant dyes
A surface-enhanced Raman scattering (SERS) substrate with good flexibility and high water absorbing capacity is reported. It consists of a calcium alginate sponge incorporating gold nanoparticles. These are in...
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Article
Dialysis assisted ligand exchange on gold nanorods: Amplification of the performance of a lateral flow immunoassay for E. coli O157:H7
Ligand exchange on the surface of gold nanorods (AuNRs) is widely used, but conventional methods usually require multiple centrifugation cycles to completely remove cetyltrimethylammonium bromide (CTAB). This ...
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Article
Open AccessAn adaptable chromosome preparation methodology for use in invertebrate research organisms
The ability to efficiently visualize and manipulate chromosomes is fundamental to understanding the genome architecture of organisms. Conventional chromosome preparation protocols developed for mammalian cells...
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Article
Highly sensitive aptamer based on electrochemiluminescence biosensor for label-free detection of bisphenol A
Bisphenol A (BPA), a typical endocrine disruptor, is widely used as a key monomer in the packaging industry. Residual monomer can transfer from the package material to the food and thereby pose a risk to the h...
