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Author Correction: The gene TaWOX5 overcomes genotype dependency in wheat genetic transformation
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Article
The gene TaWOX5 overcomes genotype dependency in wheat genetic transformation
Although great progress has been achieved regarding wheat genetic transformation technology in the past decade1–3, genotype dependency, the most impactful factor in wheat genetic transformation, currently limits ...
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Article
Expression of Arabidopsis Ornithine Aminotransferase (AtOAT) encoded gene enhances multiple abiotic stress tolerances in wheat
The drought and salt tolerances of wheat were enhanced by ectopic expression of the Arabidopsis ornithine aminotransferase (AtOAT) encoded gene. The OAT was confirmed to play a role in proline ...
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Article
Screening and functional characterization of candidate resistance genes to powdery mildew from Dasypyrum villosum#4 in a wheat line Pm97033
Three genes designated DvLox, Pm21#4, and Pm21#4-H identified in a wheat–Dasypyrum villosum#4 T6V#4S·6DL translocation line Pm97033 conferred wheat for powdery mildew resistance.
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Article
Open AccessDevelopment of PCR markers specific to Dasypyrum villosum genome based on transcriptome data and their application in breeding Triticum aestivum-D. villosum#4 alien chromosome lines
Dasypyrum villosum is an important wild species of wheat (Triticum aestivum L.) and harbors many desirable genes that can be used to improve various traits of wheat. Compared with other D. villosum accessions, D....
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Article
Development of a set of PCR markers specific to Aegilops longissima chromosome arms and application in breeding a translocation line
Transcriptome data were used to develop 134 Aegilops longissima specific PCR markers and their comparative maps were constructed by contrasting with the homo...
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Article
Development and comparative genomic map** of Dasypyrum villosum 6V#4S-specific PCR markers using transcriptome data
Twenty-five Dasypyrum villosum 6V#4S-specific PCR markers were developed using transcriptome data and further assigned to comparative genomic maps of wheat c...
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Article
Open AccessComprehensive molecular analysis of arginase-encoding genes in common wheat and its progenitor species
Arginase (ARG) contributes to nitrogen remobilization by conversion of arginine to ornithine and urea. However, wheat ARG genes have not yet been identified. Here we isolated and characterized ARG genes from whea...
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Article
Effects of the wheat UDP-glucosyltransferase gene TaUGT-B2 on Agrobacterium-mediated plant transformation
UDP-glucosyltransferase enzymes (UGTs) are known to function in plant disease defense responses. In our previous RNA-seq analysis, TaUGT expression was found to be strongly up-regulated in Agrobacterium-infected ...
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Article
Open AccessCloning and characterization of TaVIP2 gene from Triticum aestivum and functional analysis in Nicotiana tabacum
Wheat is recalcitrant to genetic transformation. A potential solution is to manipulate the expression of some host proteins involved in T-DNA integration process. VirE2 interacting protein 2 (VIP2) plays an im...
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Article
Open AccessThe wheat R2R3-MYB transcription factor TaRIM1 participates in resistance response against the pathogen Rhizoctonia cerealis infection through regulating defense genes
The necrotrophic fungus Rhizoctonia cerealis is a major pathogen of sharp eyespot that is a devastating disease of wheat (Triticum aestivum). Little is known about roles of MYB genes in wheat defense response to
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Article
Expression of a potato antimicrobial peptide SN1 increases resistance to take-all pathogen Gaeumannomyces graminis var. tritici in transgenic wheat
Take-all, caused by soil-borne fungus Gaeumannomyces graminis var. tritici (Ggt), is a devastating root disease of wheat (Triticum aestivum) worldwide. Breeding resistant wheat cultivars is the most promising and...
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Article
Efficient Regeneration Potential is Closely Related to Auxin Exposure Time and Catalase Metabolism During the Somatic Embryogenesis of Immature Embryos in Triticum aestivum L
Regeneration of cultured tissue is a prerequisite of Agrobacterium- and biolistic-mediated plant transformation. In this study, an efficient protocol for improving wheat (Triticum aestivum L.) immature embryo reg...
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Article
Combinational transformation of three wheat genes encoding fructan biosynthesis enzymes confers increased fructan content and tolerance to abiotic stresses in tobacco
Seven kinds of transgenic tobacco plants transformed with combinations of three FBE genes were obtained. The transgenic plants transformed with ...
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Article
Overexpression of wheat lipid transfer protein gene TaLTP5 increases resistances to Cochliobolus sativus and Fusarium graminearum in transgenic wheat
The fungus Cochliobolus sativus is the main pathogen of common root rot, a serious soil-borne disease of wheat (Triticum aestivum L.). The fungus Fusarium graminearum is the primary pathogen of Fusarium head blig...
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Article
Genetic transformation of wheat: current status and future prospects
Genetic transformation is a reverse genetics tool for validation of target genes and crop improvement. However, due to its low efficiency and genotype dependency, wheat is considered a recalcitrant plant for g...
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Article
Expression of a radish defensin in transgenic wheat confers increased resistance to Fusarium graminearum and Rhizoctonia cerealis
Fusarium head blight (scab), primarily caused by Fusarium graminearum, is a devastating disease of wheat (Triticum aestivum L.) worldwide. Wheat sharp eyespot, mainly caused by Rhizoctonia cerealis, is one of the...
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Article
Overexpression of TaPIEP1, a pathogen-induced ERF gene of wheat, confers host-enhanced resistance to fungal pathogen Bipolaris sorokiniana
Bipolaris sorokiniana is an economically important phytopathogen of wheat and other cereal species. In this paper, a novel pathogen-induced ethylene-responsive factor (ERF) gene of wheat, TaPIEP1, was isolated an...
