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Protocol
Dynamic Force Spectroscopy Analysis on the Redox States of Protein Disulphide Bonds
An emerging concept in chemical biology is that protein function that can be regulated by the redox state of disulphide bonds. This chapter describes the dynamic force spectroscopy method for analyzing redox r...
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Article
Open AccessApolipoprotein A-IV binds αIIbβ3 integrin and inhibits thrombosis
Platelet αIIbβ3 integrin and its ligands are essential for thrombosis and hemostasis, and play key roles in myocardial infarction and stroke. Here we show that apolipoprotein A-IV (apoA-IV) can be isolated fro...
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Article
Open AccessCompression force sensing regulates integrin αIIbβ3 adhesive function on diabetic platelets
Diabetes is associated with an exaggerated platelet thrombotic response at sites of vascular injury. Biomechanical forces regulate platelet activation, although the impact of diabetes on this process remains i...
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Article
Open AccessDual Biomembrane Force Probe enables single-cell mechanical analysis of signal crosstalk between multiple molecular species
Conventional approaches for studying receptor-mediated cell signaling, such as the western blot and flow cytometry, are limited in three aspects: 1) The perturbing preparation procedures often alter the molecu...
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Protocol
Two-Dimensional Analysis of Cross-Junctional Molecular Interaction by Force Probes
Upon engagement with a specific ligand, a cell surface receptor transduces intracellular signals to activate various cellular functions. This chapter describes a set of biomechanical methods for analyzing the ...
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Article
Open AccessMouseMove: an open source program for semi-automated analysis of movement and cognitive testing in rodents
The Open Field (OF) test is one of the most commonly used assays for assessing exploratory behaviour and generalised locomotor activity in rodents. Nevertheless, the vast majority of researchers still rely upo...
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Article
Dynamic catch of a Thy-1–α5β1+syndecan-4 trimolecular complex
Cancer cell adhesion to the vascular endothelium is a critical step of tumour metastasis. Endothelial surface molecule Thy-1 (CD90) is implicated in the metastatic process through its interactions with integri...
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Article
A new method for splice site prediction based on the sequence patterns of splicing signals and regulatory elements
It is of significance for splice site prediction to develop novel algorithms that combine the sequence patterns of regulatory elements such as enhancers and silencers with the patterns of splicing signals. In ...
