77 Result(s)
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Article
Open AccessExpression capable library for studies of Neisseria gonorrhoeae, version 1.0
The sexually transmitted disease, gonorrhea, is a serious health problem in developed as well as in develo** countries, for which treatment continues to be a challenge. The recent completion of the genome se...
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Open AccessExpression of hemagglutinin protein from the avian influenza virus H5N1 in a baculovirus/insect cell system significantly enhanced by suspension culture
Prevention of a possible avian influenza pandemic necessitates the development of rapid diagnostic tests and the eventual production of a vaccine.
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Article
Isolation and characterisation ofCandida sp. mutants enriched in S-adenosylmethionine (SAM)
The ability to accumulate S-adenosylmethionine (SAM) of 572 yeast strains isolated from environmental sources were surveyed. An S-adenosylmethionine enriching strain S42-12, identified asCandida sp., was chose to...
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Article
Cloning and expression of phospholipase D genepld fromStreptomyces chromofuscus
The phospholipase D (PLD) gene pld encoding the mature PLD enzyme fromStreptomyces chromofuscus was cloned and sequenced. The recombinant protein has been expressed inEscherichia coli and purified. The yield of a...
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Article
The efficacy of nematicidal strainSyncephalastrum racemosum
Nematicidal activity using a pure culture ofSyncephalastrum racemosum was studied. The results showed that the metabolites ofS. racemosum have pretty high nematicidal activity. The nematicidal principles were sol...
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Optimisation of fermentation medium for the detoxification of free gossypol in cottonseed powder byGeotrichum candidum G07 in solid-state fermentation with response surface methodology
Plackett-Burman design and response surface methodology were adopted to optimise the fermentation medium forGeotrichum candidum G07 to detoxify free gossypol in cottonseed powder by the solid-state fermentation. ...
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Open AccessEmodin targets the β-hydroxyacyl-acyl carrier protein dehydratase from Helicobacter pylori: enzymatic inhibition assay with crystal structural and thermodynamic characterization
The natural product Emodin demonstrates a wide range of pharmacological properties including anticancer, anti-inflammatory, antiproliferation, vasorelaxant and anti-H. pylori activities. Although its H. pylori in...
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Article
Elementary mode analysis and metabolic flux analysis of L-glutamate biosynthesis byCorynebacterium glutamicum
In this study we applied elementary mode analysis and metabolic flux analysis to L-glutamate biosynthesis byCorynebacterium glutamicum GDK-9. The theoretically optimal flux distribution with the maximal L-glutama...
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Article
Open AccessCharacterization of Zur-dependent genes and direct Zur targets in Yersinia pestis
The zinc uptake regulator Zur is a Zn2+-sensing metalloregulatory protein involved in the maintenance of bacterial zinc homeostasis. Up to now, regulation of zinc homeostasis by Zur is poorly understood in Y. pes...
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Open AccessIdentification of genes coding for B cell antigens of Mycoplasma mycoides subsp. mycoides Small Colony (Mmm SC) by using phage display
Contagious bovine pleuropneumonia (CBPP) is a mycoplasmal disease caused by Mycoplasma mycoides subsp. mycoides SC (Mmm SC). Since the disease is a serious problem that can affect cattle production in parts of Af...
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Open AccessA large scale comparative genomic analysis reveals insertion sites for newly acquired genomic islands in bacterial genomes
Bacterial virulence enhancement and drug resistance are major threats to public health worldwide. Interestingly, newly acquired genomic islands (GIs) from horizontal transfer between different bacteria strains...
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Analysis of mechanisms of resistance and tolerance of Escherichia coli to enrofloxacin
The mechanisms behind reduced bacterial sensitivity to drugs and the nature of this phenomenon were explored. Escherichia coli were treated with a sub-inhibitory dose or a repeatedly doubled dose of enrofloxacin ...
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Cloning, heterologous expression, and comparative characterization of a mesophilic α-amylase gene from Bacillus subtilis JN16 in Escherichia coli
A gene encoding mesophilic α-amylase from Bacillus subtilis JN16 was identified and designated as AmyQ. The AmyQ gene was cloned, sequenced, and expressed in Escherichia coli. AmyQ is 1,980 bp in length and encod...
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Open AccessIsolation and genomic characterization of SfI, a serotype-converting bacteriophage of Shigella flexneri
All Shigella flexneri serotypes except serotype 6 share a common O-antigen tetrasaccharide backbone and nearly all variations between serotypes are due to glucosyl and/or O-acetyl modifications of the common O un...
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Evaluating Lactobacillus and Pediococcus strains for enzyme-encoding genes related to peptide and amino acid utilization in wine
In this study, a total of 104 strains of lactic acid bacteria (LAB) were tested for the presence of genes encoding enzymes related to peptide and amino acid utilization in winemaking. Primers for PCR amplifica...
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Extracellular enzyme production and fungal mycelia degradation of antagonistic Streptomyces induced by fungal mycelia preparation of cucurbit plant pathogens
Streptomyces are beneficial soil microorganisms and potential candidates for biocontrol agents against soilborne pathogenic fungi of cucurbit plants. Extracellular enzymes such as cellulase, chit...
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Open AccessDynamics of fecal microbial communities in children with diarrhea of unknown etiology and genomic analysis of associated Streptococcus lutetiensis
The sequences of the 16S rRNA genes extracted from fecal samples provide insights into the dynamics of fecal microflora. This potentially gives valuable etiological information for patients whose conditions ha...
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Open AccessCurrent status of Staphylococcus aureus infection in a central teaching hospital in Shanghai, China
To control the spread of methicillin-resistant Staphylococcus aureus (MRSA) in hospitals, infection control measures such as hand hygiene practices were introduced into the teaching hospitals in Shanghai, China, ...
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Open AccessCharacterization of Shiga toxin-producing Escherichia coli isolated from healthy pigs in China
Shiga toxin-producing Escherichia coli (STEC) is recognized as an important human diarrheal pathogen. Swine plays an important role as a carrier of this pathogen. In this study we determined the prevalence and ch...
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Open AccessMycobacterium bovis BCG infection severely delays Trichuris muris expulsion and co-infection suppresses immune responsiveness to both pathogens
The global epidemiology of parasitic helminths and mycobacterial infections display extensive geographical overlap, especially in the rural and urban communities of develo** countries. We investigated whethe...
