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Article
Open AccessSmall leucine-rich proteoglycans inhibit CNS regeneration by modifying the structural and mechanical properties of the lesion environment
Extracellular matrix (ECM) deposition after central nervous system (CNS) injury leads to inhibitory scarring in humans and other mammals, whereas it facilitates axon regeneration in the zebrafish. However, the...
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Article
Open AccessAdipose cells and tissues soften with lipid accumulation while in diabetes adipose tissue stiffens
Adipose tissue expansion involves both differentiation of new precursors and size increase of mature adipocytes. While the two processes are well balanced in healthy tissues, obesity and diabetes type II are a...
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Article
Open AccessIsotropically resolved label-free tomographic imaging based on tomographic moulds for optical trap**
A major challenge in three-dimensional (3D) microscopy is to obtain accurate spatial information while simultaneously kee** the microscopic samples in their native states. In conventional 3D microscopy, axia...
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Article
Open AccessRecent progress and current opinions in Brillouin microscopy for life science applications
Many important biological functions and processes are reflected in cell and tissue mechanical properties such as elasticity and viscosity. However, current techniques used for measuring these properties have m...
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Article
Open AccessStudy of Optical Configurations for Multiple Enhancement of Microalgal Biomass Production
Microalga is a promising biomass feedstock to restore the global carbon balance and produce sustainable bioenergy. However, the present biomass productivity of microalgae is not high enough to be marketable ma...
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Article
Open AccessSuper-resolution three-dimensional fluorescence and optical diffraction tomography of live cells using structured illumination generated by a digital micromirror device
We present a multimodal approach for measuring the three-dimensional (3D) refractive index (RI) and fluorescence distributions of live cells by combining optical diffraction tomography (ODT) and 3D structured ...
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Article
Open AccessMelittin-induced alterations in morphology and deformability of human red blood cells using quantitative phase imaging techniques
Here, the actions of melittin, the active molecule of apitoxin or bee venom, were investigated on human red blood cells (RBCs) using quantitative phase imaging techniques. High-resolution real-time 3-D refract...
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Article
Open AccessIdentification of non-activated lymphocytes using three-dimensional refractive index tomography and machine learning
Identification of lymphocyte cell types are crucial for understanding their pathophysiological roles in human diseases. Current methods for discriminating lymphocyte cell types primarily rely on labelling tech...
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Article
Open AccessTomographic active optical trap** of arbitrarily shaped objects by exploiting 3D refractive index maps
Optical trap** can manipulate the three-dimensional (3D) motion of spherical particles based on the simple prediction of optical forces and the responding motion of samples. However, controlling the 3D behav...
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Article
Open AccessRefractive index tomograms and dynamic membrane fluctuations of red blood cells from patients with diabetes mellitus
In this paper, we present the optical characterisations of diabetic red blood cells (RBCs) in a non-invasive manner employing three-dimensional (3-D) quantitative phase imaging. By measuring 3-D refractive ind...
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Article
Open AccessThree-dimensional label-free imaging and quantification of lipid droplets in live hepatocytes
Lipid droplets (LDs) are subcellular organelles with important roles in lipid storage and metabolism and involved in various diseases including cancer, obesity, and diabetes. Conventional methods, however, hav...
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Article
Open AccessMeasuring cell surface area and deformability of individual human red blood cells over blood storage using quantitative phase imaging
The functionality and viability of stored human red blood cells (RBCs) is an important clinical issue in transfusions. To systematically investigate changes in stored whole blood, the hematological properties ...
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Article
Open AccessHolographic intravital microscopy for 2-D and 3-D imaging intact circulating blood cells in microcapillaries of live mice
Intravital microscopy is an essential tool that reveals behaviours of live cells under conditions close to natural physiological states. So far, although various approaches for imaging cells in vivo have been pro...
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Article
Open AccessOptical characterization of red blood cells from individuals with sickle cell trait and disease in Tanzania using quantitative phase imaging
Sickle cell disease (SCD) is common across Sub-Saharan Africa. However, the investigation of SCD in this area has been significantly limited mainly due to the lack of research facilities and skilled personnel....
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Article
Open AccessLabel-free optical quantification of structural alterations in Alzheimer’s disease
We present a wide-field quantitative label-free imaging of mouse brain tissue slices with sub-micrometre resolution, employing holographic microscopy and an automated scanning platform. From the measured light...
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Article
Open AccessCharacterizations of individual mouse red blood cells parasitized by Babesia microti using 3-D holographic microscopy
Babesia microti causes “emergency” human babesiosis. However, little is known about the alterations in B. microti invaded red blood cells (Bm-RBCs) at the individual cell level. Through quantitative phase imaging...
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Article
Open AccessProfiling individual human red blood cells using common-path diffraction optical tomography
Due to its strong correlation with the pathophysiology of many diseases, information about human red blood cells (RBCs) has a crucial function in hematology. Therefore, measuring and understanding the morpholo...
