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Article
Characterization and arbovirus susceptibility of cultured CERNI cells derived from sika deer (Cervus nippon)
Cervus nippon (sika deer) are widely distributed throughout eastern Asia. Deer possess a variety of antibodies against several zoonotic pathogens, indicating that they act as reservoir of zoonoses. In this study...
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Article
Open AccessConstruction of Vero cell-adapted rabies vaccine strain by five amino acid substitutions in HEP-Flury strain
Rabies virus (RABV) causes fatal neurological disease. Pre-exposure prophylaxis (PrEP) and post-exposure prophylaxis (PEP) using inactivated-virus vaccines are the most effective measures to prevent rabies. In...
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Article
Guidance for performance, utilization, and education of cardiac and lung point-of-care ultrasonography from the Japanese Society of Echocardiography
In recent years, bedside ultrasound examinations have been used in many clinical departments and are called point-of-care ultrasound (POCUS). Regarding POCUS in the cardiac field, a protocol called focus (focu...
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Article
Open AccessGn protein expressed in plants for diagnosis of severe fever with thrombocytopenia syndrome virus
Severe fever with thrombocytopenia syndrome virus (SFTSV) causes the highly fatal disease in humans. To facilitate diagnosis, the native form of subunit glycoprotein (Gn), a prime target for potential vaccines...
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Article
Open AccessCharacterization of Omicron BA.4.6, XBB, and BQ.1.1 subvariants in hamsters
During the Omicron wave, previous variants such as BA.2, BA.4, and BA.5 were replaced by newer variants with additional mutations in the spike protein. These variants, BA.4.6, BQ.1.1, and XBB, have spread in d...
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Article
Establishment and characterization of a novel lung cell line derived from the common bottlenose dolphin
Cetaceans are specialized marine mammals with a unique respiratory system adapted for diving behavior. Furthermore, respiratory diseases are commonly observed in these mammals. Nevertheless, much of their resp...
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Article
Open AccessStructural delineation and computational design of SARS-CoV-2-neutralizing antibodies against Omicron subvariants
SARS-CoV-2 Omicron subvariants have evolved to evade receptor-binding site (RBS) antibodies that exist in diverse individuals as public antibody clones. We rationally selected RBS antibodies resilient to mutat...
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Article
Open AccessCharacterization of SARS-CoV-2 Omicron BA.2.75 clinical isolates
The prevalence of the Omicron subvariant BA.2.75 rapidly increased in India and Nepal during the summer of 2022, and spread globally. However, the virological features of BA.2.75 are largely unknown. Here, we ...
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Article
Characterization of SARS-CoV-2 Omicron BA.4 and BA.5 isolates in rodents
The BA.2 sublineage of the SARS-CoV-2 Omicron variant has become dominant in most countries around the world; however, the prevalence of BA.4 and BA.5 is increasing rapidly in several regions. BA.2 is less pat...
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Article
Open AccessEssential role of TMPRSS2 in SARS-CoV-2 infection in murine airways
In cultured cells, SARS-CoV-2 infects cells via multiple pathways using different host proteases. Recent studies have shown that the furin and TMPRSS2 (furin/TMPRSS2)-dependent pathway plays a minor role in in...
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Article
Characterization and antiviral susceptibility of SARS-CoV-2 Omicron BA.2
The recent emergence of SARS-CoV-2 Omicron (B.1.1.529 lineage) variants possessing numerous mutations has raised concerns of decreased effectiveness of current vaccines, therapeutic monoclonal antibodies and a...
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Article
Isolation and whole-genome sequencing of a novel aviadenovirus from owls in Japan
Adenoviruses have been reported to infect a variety of birds. Here, we isolated a novel adenovirus from the liver of a dead owl chick (Bengal eagle owl; Bubo bengalensis) at a raptor-breeding facility in Japan an...
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Article
Distinct interferon response in bat and other mammalian cell lines infected with Pteropine orthoreovirus
Bats serve as natural hosts of Pteropine orthoreovirus (PRV), an emerging group of bat-borne, zoonotic viruses. Bats appear to possess unique innate immune system responses that can inhibit viral replication, thu...
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Article
Open AccessACE2-like carboxypeptidase B38-CAP protects from SARS-CoV-2-induced lung injury
Angiotensin-converting enzyme 2 (ACE2) is a receptor for cell entry of SARS-CoV-2, and recombinant soluble ACE2 protein inhibits SARS-CoV-2 infection as a decoy. ACE2 is a carboxypeptidase that degrades angiot...
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Article
Open AccessA novel nairovirus associated with acute febrile illness in Hokkaido, Japan
The increasing burden of tick-borne orthonairovirus infections, such as Crimean-Congo hemorrhagic fever, is becoming a global concern for public health. In the present study, we identify a novel orthonairoviru...
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Article
Open AccessThe evolution of hard tick-borne relapsing fever borreliae is correlated with vector species rather than geographical distance
Relapsing fever (RF) borreliae are arthropod-borne spirochetes and some of them cause human diseases, which are characterized by relapsing or recurring episodes of fever. Recently, it has been classified into ...
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Article
Antiviral effect of sinefungin on in vitro growth of feline herpesvirus type 1
Feline herpesvirus type 1 (FHV-1) causes a potentially fatal disease in cats. Through the use of virus inhibition and cytotoxicity assays, sinefungin, a nucleoside antibiotic, was assessed for its potential to...
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Article
Open AccessSevere Fever with Thrombocytopenia Syndrome Phlebovirus causes lethal viral hemorrhagic fever in cats
Severe fever with thrombocytopenia syndrome (SFTS) is an emerging hemorrhagic fever caused by the SFTS phlebovirus (SFTSV). SFTS patients were first reported in China, followed by Japan and South Korea. In 201...
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Article
Open AccessBloodmeal host identification with inferences to feeding habits of a fish-fed mosquito, Aedes baisasi
The mosquito, Aedes baisasi, which inhabits brackish mangrove swamps, is known to feed on fish. However, its host assemblage has not been investigated at the species level. We amplified and sequenced the cytochro...
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Article
Efficacy of a novel mixture of substances derived from food and food additives for controlling Dermanyssus gallinae (Mesostigmata: Dermanyssidae)
Dermanyssus gallinae De Geer (Mesostigmata: Dermanyssidae), also known as poultry red mite, causes serious economic losses to the poultry industry worldwide. In this study, a mixture of substances derived from fo...
