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Article
Hybrid composite cellulose nanocrystal, hydroxyapatite, and chitosan material with controlled hydrophilic/hydrophobic properties as a remineralizable dental material
It is expected that cellulose nanocrystals (CNCs) will be utilized for biomedical applications, especially hard tissue engineering, because of their low toxicity to the human body and exceptional physicochemic...
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Article
Open AccessConvergent use of phosphatidic acid for hepatitis C virus and SARS-CoV-2 replication organelle formation
Double membrane vesicles (DMVs) serve as replication organelles of plus-strand RNA viruses such as hepatitis C virus (HCV) and SARS-CoV-2. Viral DMVs are morphologically analogous to DMVs formed during autopha...
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Article
Biocompatible composite of cellulose nanocrystal and hydroxyapatite with large mechanical strength
Exploring the simple fabrication process to prepare CNC@HAp for biological tissues is still a challenging subject considering the wide applications of the composites for bio tissues. In this work, aiming for t...
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Article
Open AccessAge-dependent loss of adipose Rubicon promotes metabolic disorders via excess autophagy
The systemic decline in autophagic activity with age impairs homeostasis in several tissues, leading to age-related diseases. A mechanistic understanding of adipocyte dysfunction with age could help to prevent...
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Article
ER-sha** atlastin proteins act as central hubs to promote flavivirus replication and virion assembly
Flaviviruses, including dengue virus and Zika virus, extensively remodel the cellular endomembrane network to generate replication organelles that promote viral genome replication and virus production. However...
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Article
Open AccessGalactosyl carbohydrate residues on hematopoietic stem/progenitor cells are essential for homing and engraftment to the bone marrow
The role of carbohydrate chains in leukocyte migration to inflamed sites during inflammation and trafficking to the lymph nodes under physiological conditions has been extensively characterized. Here, we repor...
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Protocol
Immuno-localization of ESCRT Proteins in Virus-Infected Cells by Fluorescence and Electron Microscopy
Many enveloped viruses utilize the cellular ESCRT pathway for budding, even flaviviruses, which form viral particles inside replication organelles derived from the endoplasmic reticulum (ER). In this section, ...
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Protocol
Morphological Analysis of Autophagy
Autophagy is a bulk intracellular degradation process that is ubiquitous in eukaryotic cells and helps to recycle nutrients from catabolites by degrading proteins, lipids, and glycans, including organelles. Si...
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Article
Two Beclin 1-binding proteins, Atg14L and Rubicon, reciprocally regulate autophagy at different stages
Beclin 1 is an essential mediator of mammalian autophagy that has anti-tumour activity. Beclin 1 associates with Atg14L and Rubicon to regulate autophagosome formation and maturation, respectively.
