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Article
Effective Application of DAS-ELISA for Detection of Grapevine Leafroll Associated Closterovirus-3 Using a Polyclonal Antiserum Developed from Recombinant Coat Protein
A polyclonal antiserum (As163) specific to grapevine leafroll associated closterovirus-3 (GLRaV-3) was developed using a recombinant coat protein expressed in E. coli from a cDNA clone identified after immunoscre...
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Article
Assessment of the phytotoxicity of chromium in soils using the selective ion exchange resin extraction method
Chromium in soils is present in the form of Cr(VI) oxyanions or Cr(III) cations. The toxicity and mobility of Cr(VI) are higher than those of Cr(III), thus it is essential that the availability of Cr(VI) in so...
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Article
Molecular Cloning and Expression of IL2 cDNA from the Tibet Pig
Interleukin-2 is a vital cytokine secreted by activated T lymphocytes, and plays important role in the regulation of cellular and humoral immunity of animals. In our experiment, IL2 cDNA of the Tibet Pig was f...
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Article
Assessment of Phytotoxicity of Chromium in Flooded Soils using Embedded Selective Ion Exchange Resin Method
Chromium present in the forms of Cr(VI) or Cr(III) in soils. Since the toxicity and mobility of Cr(VI) are higher than those of Cr(III), it would be important to estimate soil Cr(VI) accurately in order to ass...
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Article
Using the Selective Ion Exchange Resin Extraction and XANES Methods to Evaluate the Effect of Compost Amendments on Soil Chromium(VI) Phytotoxicity
The Cu-saturated selective ion exchange resin (DOWEX M4195) extraction method was used to investigate the effects of two amendments, 5 and 15% organic matter in the form of hog-dung compost (HC) or cattle-dung...
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Article
Open AccessGlycogen synthase kinase 3α and 3β have distinct functions during cardiogenesis of zebrafish embryo
Glycogen synthase kinase 3 (GSK3) encodes a serine/threonine protein kinase, is known to play roles in many biological processes. Two closely related GSK3 isoforms encoded by distinct genes: GSK3α (51 kDa) and...
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Article
Open AccessPopulation phylogenomic analysis of mitochondrial DNA in wild boars and domestic pigs revealed multiple domestication events in East Asia
Previously reported evidence indicates that pigs were independently domesticated in multiple places throughout the world. However, a detailed picture of the origin and dispersal of domestic pigs in East Asia h...
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Article
A plant nutrition strategy for ex-situ conservation based on “Ecological Similarity”
This paper reviewed a large scale conservation work of rare and endangered plants currently conducted in main botanical gardens in China, and the existed, predictable and neglected problems on plant growth and...
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Article
Protection against Helicobacter pylori infection by a trivalent fusion vaccine based on a fragment of urease B-UreB414
A multivalent fusion vaccine is a promising option for protection against Helicobacter pylori infection. In this study, UreB414 was identified as an antigenic fragment of urease B subunit (UreB) and it induced an...
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Article
Assessing the phytotoxicity of chromium in Cr(VI)-spiked soils by Cr speciation using XANES and resin extractable Cr(III) and Cr(VI)
The phytotoxicity of soil Cr usually depends on the plant availability of Cr(VI) in Cr-contaminated soils. However, Cr(VI) is favorably reduced to Cr(III) under acidic conditions, and increased availability of...
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Article
A systemic proteomic analysis of Populus chloroplast by using shotgun method
The chloroplast is one of the most important organelles in plants. Proteomic investigations of chloroplasts have been undertaken for many herb plant species, but to date no such investigation has been reported...
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Article
Open AccessMusashi1 expression cells derived from mouse embryonic stem cells can be enriched in side population isolated by fluorescence activated cell sorter
Purifying stem cells is an inevitable process for further investigation and cell-therapy. Sorting side population (SP) cells is generally regarded as an effective method to enrich for progenitor cells. This st...
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Article
Isolation of a buprofezin co-metabolizing strain of Pseudomonas sp. DFS35-4 and identification of the buprofezin transformation pathway
Buprofezin is a widely used insecticide that has caused environmental pollution in many areas. However, biodegradation of buprofezin by pure cultures has not been extensively studied, and the transformation pa...
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Article
Indole alkaloids from marine-derived fungus Aspergillus sydowii SCSIO 00305
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Article
Lysyl oxidase polymorphisms and ischemic stroke—a case control study
Ischemic stroke is a common neurological disease and causes severe disability and death worldwide. Lysyl oxidase (LOX) plays a crucial role in the maintenance of extracellular matrix stability and may particip...
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Article
Optimization of Cultivating Conditions for Triterpenoids Production from Antrodia cinnmomea
The submerged cultivating conditions for triterpenoids production from Antrodia cinnamomea were optimized using uniform design method and the one-factor-at-a-time method was adopted to investigate the effect of p...
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Article
Open AccessSpace-related pharma-motifs for fast search of protein binding motifs and polypharmacological targets
To discover a compound inhibiting multiple proteins (i.e. polypharmacological targets) is a new paradigm for the complex diseases (e.g. cancers and diabetes). In general, the polypharmacological proteins often...
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Article
A functional variant in the cystathionine β-synthase gene promoter significantly reduces congenital heart disease susceptibility in a Han Chinese population
Homocysteine is an independent risk factor for various cardiovascular diseases. There are two ways to remove homocysteine from embryonic cardiac cells: remethylation to form methionine or transsulfuration to f...
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Article
Open AccessPost-translational regulation enables robust p53 regulation
The tumor suppressor protein p53 plays important roles in DNA damage repair, cell cycle arrest and apoptosis. Due to its critical functions, the level of p53 is tightly regulated by a negative feedback mechani...
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Article
Open AccessCochaperonin CPN20 negatively regulates abscisic acid signaling in Arabidopsis
Previous study showed that the magnesium-protoporphyrin IX chelatase H subunit (CHLH/ABAR) positively regulates abscisic acid (ABA) signaling. Here, we investigated the functions of a CHLH/ABAR interaction pro...