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  1. Article

    Open Access

    Indel locations are determined by template polarity in highly efficient in vivo CRISPR/Cas9-mediated HDR in Atlantic salmon

    Precise gene editing such as CRISPR/Cas9-mediated homology directed repair (HDR) can increase our understanding of gene function and improve traits of importance for aquaculture. This fine-tuned technology has...

    Anne Hege Straume, Erik Kjærner-Semb, Kai Ove Skaftnesmo in Scientific Reports (2020)

  2. Article

    Open Access

    Sex steroid production associated with puberty is absent in germ cell-free salmon

    In all vertebrates studied so far, germ cells are not required for pubertal maturation of the gonadal steroidogenic system, subsequent development of secondary sex characteristics and reproductive behavior. To...

    Lene Kleppe, Eva Andersson, Kai Ove Skaftnesmo, Rolf B. Edvardsen in Scientific Reports (2017)

  3. Article

    Open Access

    Dnd knockout ablates germ cells and demonstrates germ cell independent sex differentiation in Atlantic salmon

    Introgression of farmed salmon escapees into wild stocks is a major threat to the genetic integrity of wild populations. Using germ cell-free fish in aquaculture may mitigate this problem. Our study investigat...

    Anna Wargelius, Sven Leininger, Kai Ove Skaftnesmo, Lene Kleppe in Scientific Reports (2016)