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Article
Open AccessSingle nucleotide replacement in the Atlantic salmon genome using CRISPR/Cas9 and asymmetrical oligonucleotide donors
New breeding technologies (NBT) using CRISPR/Cas9-induced homology directed repair (HDR) has the potential to expedite genetic improvement in aquaculture. The long generation time in Atlantic salmon makes bree...
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Article
Open AccessInsulin-like 3 affects zebrafish spermatogenic cells directly and via Sertoli cells
Pituitary hormones can use local signaling molecules to regulate target tissue functions. In adult zebrafish testes, follicle-stimulating hormone (Fsh) strongly increases the production of insulin-like 3 (Insl...
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Article
Open AccessTranscriptomic analysis of dead end knockout testis reveals germ cell and gonadal somatic factors in Atlantic salmon
Sustainability challenges are currently hampering an increase in salmon production. Using sterile salmon can solve problems with precocious puberty and genetic introgression from farmed escapees to wild popula...
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Article
Open AccessIndel locations are determined by template polarity in highly efficient in vivo CRISPR/Cas9-mediated HDR in Atlantic salmon
Precise gene editing such as CRISPR/Cas9-mediated homology directed repair (HDR) can increase our understanding of gene function and improve traits of importance for aquaculture. This fine-tuned technology has...
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Article
Open AccessInhibition of mitochondrial respiration prevents BRAF-mutant melanoma brain metastasis
Melanoma patients carry a high risk of develo** brain metastases, and improvements in survival are still measured in weeks or months. Durable disease control within the brain is impeded by poor drug penetrat...
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Article
Open AccessSex steroid production associated with puberty is absent in germ cell-free salmon
In all vertebrates studied so far, germ cells are not required for pubertal maturation of the gonadal steroidogenic system, subsequent development of secondary sex characteristics and reproductive behavior. To...
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Article
Open AccessDnd knockout ablates germ cells and demonstrates germ cell independent sex differentiation in Atlantic salmon
Introgression of farmed salmon escapees into wild stocks is a major threat to the genetic integrity of wild populations. Using germ cell-free fish in aquaculture may mitigate this problem. Our study investigat...
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Article
Open AccessA co-culture model with brain tumor-specific bioluminescence demonstrates astrocyte-induced drug resistance in glioblastoma
Although several studies suggest that stromal fibroblasts mediate treatment resistance in several cancer types, little is known about how tumor-associated astrocytes modulate the treatment response in brain tu...
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Article
Open AccessEGFR wild-type amplification and activation promote invasion and development of glioblastoma independent of angiogenesis
Angiogenesis is regarded as a hallmark of cancer progression and it has been postulated that solid tumor growth depends on angiogenesis. At present, however, it is clear that tumor cell invasion can occur with...
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Article
Open AccessNeuronal markers are expressed in human gliomas and NSE knockdown sensitizes glioblastoma cells to radiotherapy and temozolomide
Expression of neuronal elements has been identified in various glial tumors, and glioblastomas (GBMs) with neuronal differentiation patterns have reportedly been associated with longer survival. However, the n...
