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Article
Phospholipases: Occurrence and production in microorganisms, assay for high-throughput screening, and gene discovery from natural and man-made diversity
Various kinds of phospholipids have wide industrial applications such as in food and nutraceuticals, cosmetics, agricultural products, and pharmaceuticals. The demand for reliable biocatalysts for the producti...
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Article
Hydrolysis of soybean isoflavone glucosides by lactic acid bacteria
Lactobacillus delbrueckii subsp. delbrueckii KCTC 1047, grown in de Man, Rogosa and Sharpe (MRS) or soymilk media, completely hydrolyzed the isoflavone glucosides, genistin and daidzin at 50 μg ml−1, into their ...
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Article
Enzymatic synthesis of low-calorie structured lipids in a solvent-free system
For the synthesis of low-calorie structured lipids (LCSL), transesterification between triacetin and stearic acid using immobilized lipase in a solvent-free system was investigated. Stearic acid, a long-chain ...
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Protocol
Water Activity Control for Lipase-Catalyzed Reactions in Nonaqueous Media
Lipase can be used for the synthetic reaction if the reaction is carried out in low-water media. The synthetic yield can be enhanced by continuously removing the water produced during the reaction. Many resear...
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Article
The effects of roasting temperatures on the formation of headspace volatile compounds in perilla seed oil
Volatile compounds of perilla seed oils roasted at different temperatures (150–190°C) were analyzed by dynamic headspace gas chromatography-mass spectrometry. The headspace volatiles in roasted perilla seed oi...
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Article
Stabilization of papain and lysozyme for application to cosmetic products
For cosmetic applications, papain or lysozyme was conjugated to a soluble biopolymer produced by Schizophyllum commune. Stability of the conjugated enzymes were significantly enhanced, such that more than 90% of ...
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Article
Transesterification of phosphatidylcholine with eicosapentaenoic acid ethyl ester using phospholipase A2 in organic solvent
Phospholipase A2-catalysed transesterification of phosphatidylcholine (PC, 99%) with eicosapentaenoic acid ethyl ester (EPAEE, 95%) was carried out in organic solvent. The maximum yield was 14.3% (w/w). The optim...
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Article
Concentration of bovine plasmalogen using phospholipase A1 in two-phase system
Plasmalogen that contains choline was concentrated from bovine heart to 95% purity from an initial value of 45%, and ethanolamine-containing plasmalogen from bovine brain to 99% purity from initial 64% using p...
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Article
Optimization of reaction variables for sucrose monoester production using lipase in a solvent free system by Taguchi's method
Taguchi's method was attempted successfully to optimize the reaction variables and their ranges for sucrose monoester production. Optimal conditions determined by Taguchi's method were 35°C, 200rpm, capric aci...
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Article
Simple and rapid screening methods for microorganisms with phospholipase A1, A2 and C activities
A simple and rapid screening method for microorganisms with phospholipase A1, A2 and C activities using agar plate and gas chromatography (GC) method was successfully carried out. In agar plate method, soy bean l...
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Article
A rapid gas chromatography method for quantitation of free fatty acids, monoacyl-, diacyl-, and triacylglycerols without derivatization
Quantitative analysis of mixtures of free fatty acids, monoacyl-, diacyl-, and triacylglycerols by a simple and rapid gas chromatography method has been developed. The analysis was carried out on a short capil...
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Article
Controlled mycelial growth for kojic acid production using Ca-alginate-immobilized fungal cells
Conidia of Aspergillus oryzae were immobilized in Ca-alginate beads and then incubated in a nutrient medium to yield an immobilized biocatalyst producing kojic acid. The immobilized cell cultures produced kojic a...
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Chapter and Conference Paper
Reconsideration of Commercially Available Medium Component in Recombinant Yeast Culture
There have been many reports on the use of YNB w/o AA in the culture medium for recombinant yeast (1-5). Park et al. (6) and Gu et al. (7) had also used YNB w/o AA to produce Hepatitis B surface antigen (HBsAg...
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Article
Supplement of nutrients for effective cultivation of hepatitis B surface antigen-producing recombinant yeast
In the hepatitis B surface antigen (HBsAg)-producing recombinant yeast culture medium, the supply of Bacto-yeast nitrogen base without amino acids was found to be inadequate due to the lack of the several kind...
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Article
Stability of the lipase immobilized on DEAE-Sephadex for continuous lipid hydrolysis in organic solvent
Continuous hydrolysis reaction was carried out in glass-column by using lipase fromCandida rugosa immobilized on DEAE-Sephadex A50. Substrate olive oil dissolved in hydrophobic organic solvent (isooctane) was sup...
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Article
Overexpression of a thermostable lipase gene from Pseudomonas fluorescens in Escherichia coli
A thermostable lipase gene from Pseudomonas fluorescens SIK W1 was overexpressed in Escherichia coli BL21 using expression vector pTTY2. The amount of lipase produced by E. coli BL21 with pTTY2 was more than 40% ...
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Article
Effect of solvents on hydrolysis of olive oil by immobilized lipase in reverse phase system
Lipase fromCandida rugosa was immobilized by adsorption on three supports which could contain water available for the hydrolysis of olive oil in a reverse phase system. To select the most suitable solvent for thi...
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Article
Effect of water on hydrolysis of olive oil by immobilized lipase in reverse phase system
Candida rugosa lipase immobilized by adsorption on the swollen Sephadex LH-20 and Sephadex LH-60 could effectively hydrolyze olive oil at high concentration in a reverse phase system. Initial water content was f...
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Article
Physiological constraints in increasing biomass concentration ofEscherichia coli B in fed-batch culture
Fed-batch culture ofE.coli B was carried out to obtain high concentration of biomass. After requirement of oxygen was met by sparging the pure oxygen, physiological constraints were delineated. High partial press...
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Article
High density cell culture ofRhodotorula glutinis using oxygen-enriched air
Fed-batch culture ofRhodotorula glutinis, an oleaginous yeast, was carried out to obtain high biomass concentration. With air, final concentration of biomass was 100–110 g/L, whereas with oxygen-enriched air, 185...
