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Article
Plant-made pharmaceuticals: exploring studies for the production of recombinant protein in plants and assessing challenges ahead
The production of pharmaceutical compounds in plants is attracting increasing attention, as plant-based systems can be less expensive, safer, and more scalable than mammalian, yeast, bacterial, and insect cell...
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Article
Accumulation of γ-linolenic acid and stearidonic acid in rapeseeds that heterologously express the Phytophthora citrophthora Δ6 desaturase gene
Most oilseeds contain polyunsaturated fatty acids, such as linoleic acid (LA) and α-linolenic acid (ALA) that are important essential fatty acids for human health. γ-Linolenic acid (GLA) and stearidonic acid (SDA...
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Article
Vegetable oil production in vegetative plant tissues
Vegetable oils, which are stored in seeds as triacylglycerol (TAG), represent a valuable source of food, fuel, and industrial raw materials. Given the increasing demand for vegetable oils, it is essential to i...
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Article
Functional Characterization of Polyunsaturated Fatty Acid Delta 6-Desaturase and Elongase Genes from the Black Seabream (Acanthopagrus schlegelii)
Fatty acid delta 6-desaturase (D6DES) and elongases are key enzymes in the synthesis of polyunsaturated fatty acids (PUFAs) including arachidonic acid (ARA) and eicosapentaenoic acid (EPA) from microorganisms ...
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Article
Identification and functional characterization of polyunsaturated fatty acid elongase (McELOVL5) gene from pike eel (Muraenesox cinereus)
The cDNA coding for a polyunsaturated fatty acid elongase (McELOVL5) was isolated from the brain of the pike eel (Muraenesox cinereus) being based on available sequences in 23 types of fish. Four sequence variant...
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Article
Isolation and functional characterization of a delta 6-desaturase gene from the pike eel (Muraenesox cinereus)
Stearidonic acid (STA; 18:4n-3) and γ-linolenic acid (GLA; 18:3n-6) are significant intermediates in the biosynthetic pathway for the very-long-chain polyunsaturated fatty acids of eicosapentaenoic acid (EPA; ...
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Article
Isolation and functional characterization of polyunsaturated fatty acid elongase (AsELOVL5) gene from black seabream (Acanthopagrus schlegelii)
To identify the genes encoding fatty acid elongases for the biosynthesis of polyunsaturated fatty acids (PUFAs), we isolated a cDNA via degenerate PCR and RACE-PCR from Acanthopagrus schlegelii with a high simila...
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Article
Functional characterization of a delta 6-desaturase gene from the black seabream (Acanthopagrus schlegeli)
Delta 6-fatty acid desaturase (D6DES) is used in the synthesis of polyunsaturated fatty acids (PUFAs) from microorganisms to higher animals, including arachidonic acid (ARA) and eicosapentaenoic acid (EPA). A ...
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Article
Proteomic identification of toxic volatile organic compound-responsive proteins in Arabidopsis thaliana
The proteins that are responsive to toxic volatile organic compounds (VOC) such as formaldehyde and toluene were analyzed with proteome analysis using two-dimensional difference image gel electrophoresis (DIGE...
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Article
Open AccessArabidopsis R2R3-MYB transcription factor AtMYB60 functions as a transcriptional repressor of anthocyanin biosynthesis in lettuce (Lactuca sativa)
The MYB transcription factors play important roles in the regulation of many secondary metabolites at the transcriptional level. We evaluated the possible roles of the Arabidopsis R2R3-MYB transcription factors i...
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Article
Genes up-regulated during red coloration in UV-B irradiated lettuce leaves
Molecular analysis of gene expression differences between green and red lettuce leaves was performed using the SSH method. BlastX comparisons of subtractive expressed sequence tags (ESTs) indicated that 7.6% o...
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Article
Identification of differentially expressed genes involved in spine formation on seeds ofDaucus carota L. (carrot), using annealing control primer (ACP) system
Carrot seeds normally have surface spines. The availability of a spineless mutant would be agronomically beneficial, eliminating the current efforts to remove those spines pre-sowing. Furthermore, the identifi...
