38 Result(s)
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Article
Binary vectors for efficient transformation of rice
We constructed binary vectors that were designed for transfer and expression of a gene into rice chromosomes. The binary vectors contained the hygromycin-resistance gene for selection of transformants and mult...
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Article
Production of transgenic rice plants showing reduced heading date and plant height by ectopic expression of rice MADS-box genes
Plant architecture is an important factor that controls several characteristics, such as light interception, harvest index, and lodging. Plant architecture is controlled by a group of regulatory genes. It was ...
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Article
A complete sequence of the pGA1611 binary vector
We report the nucleotide sequence of the binary vector pGA1611, which is used for the transformation of foreign DNA into rice. This vector is 13,476 bp long. The 5577- bp T- DNA region consists of a 1987- bp u...
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Article
Analysis of carbon metabolism in transgenicArabidopsis thaliana transformed with the cyanobacterial sucrose phosphate synthase gene
To investigate the effects of sucrose-phosphate synthase (SPS) on carbon partitioning, transgenicArabidopsis plants transformed withSynechocystis SPS were constructed. The integration, copy number and expression ...
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Article
Carbon-partitioning inArabidopsis is regulated by the fructose 6-phosphate, 2-kinase/fructose 2,6-bisphosphatase enzyme
To further elucidate the mechanisms underlying carbon-partitioning in plants, we established an experimental system by generating transgenicArabidopsis lines that overexpress both the fructose 6-phosphate, 2-kina...
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Article
Two novel protein kinase genes,OsMSRPK1 andOsMSURPK2, are regulated by diverse environmental stresses in rice
Two novel rice (Oryza sativa L.) protein kinase (PK) genes have been isolated.OsMSRPK1 andOsMSURPK2, which most likely exist as single-copy genes in the rice genome, encode 693 and S03 amino acids polypeptide, re...
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Protocol
Use of Nipponbare BAC Clones for Physical Map** of an R Gene Locus in Rice
Major advances in rice genomics during the last few years have made positional cloning in rice much more efficient. Nipponbare is a model rice genotype being sequenced by the International Rice Genome Sequenci...
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Article
Altered sucrose synthesis in rice plants with reduced activity of fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase
Fructose-6-phosphate 2-kinase/fructose-2,6-bisphosphatase (F2KP) is a bifunctional enzyme involved in regulating photosynthetic carbon metabolism by modulating the cellular level of fructose-2,6-bisphosphate (...
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Article
The identification of candidate rice genes that confer resistance to the brown planthopper (Nilaparvata lugens) through representational difference analysis
The development of rice varieties (Oryza sativa L.) that are resistant to the brown planthopper (BPH; Nilaparvata lugens Stål) is an important objective in current breeding programs. In this study, we generated 1...
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Article
The HSP90-SGT1-RAR1 molecular chaperone complex: A core modulator in plant immunity
The HSP90 (heat shock protein 90), SGT1 (suppressor of G-two allele ofSkp1), and RAR1 (required forMla12 resistance) proteins in plants form a molecular chaperone complex which is involved in diverse biological s...
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Article
The effect of DTT in protein preparations for proteomic analysis: Removal of a highly abundant plant enzyme, ribulose bisphosphate carboxylase/oxygenase
Rubisco is a major photosynthetic plant enzyme in the chloroplasts, catalyzing a photosynthetic reaction through carboxylation and oxygenation in the leaves. Despite its biological importance, its high abundan...
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Article
Molecular tagging of the Bph1 locus for resistance to brown planthopper (Nilaparvata lugens Stål) through representational difference analysis
During brown planthopper (BPH) feeding on rice plants, we employed a modified representational difference analysis (RDA) method to detect rare transcripts among those differentially expressed in SNBC61, a BPH res...
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Chapter and Conference Paper
Resistance Genes and Their Effects to Blast in Korean Rice Varieties (Oryza sativa L.)
A total of 98 varieties consisting of 88 japonica and 10 Tongil-type of rice were analyzed to resistant genes and their effects to blast. The 13 major blast resistance (R) genes against Magnaporthe oryzae were sc...
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Article
Molecular characterization and physico-chemical analysis of a new giant embryo mutant allele (ge t) in rice (Oryza sativa L.)
The rice embryo is rich in lipid and protein bodies, bioactive chemicals such as dietary fiber, phytic acids, vitamin B and E, and gamma aminobutyric acid (GABA) than the endosperm. In this paper, we report a ...
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Protocol
Development of an Efficient Inverse PCR Method for Isolating Gene Tags from T-DNA Insertional Mutants in Rice
The central goal of current genomics research in plants, as in other organisms, is to elucidate the functions of every gene. Insertional mutagenesis using known DNA sequences such as T-DNA is a powerful tool i...
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Article
Open AccessGenetic and Molecular Insights into the Enhancement of Rice Yield Potential
Rice is one of the most important global food crops and a primary source of calories for more than half of the world's population. Rice production increased steadily during the green revolution era primarily a...
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Article
Web Tools for Rice Transcriptome Analyses
Gene expression databases provide profiling data for the expression of thousands of genes to researchers worldwide. Oligonucleotide microarray technology is a useful tool that has been employed to produce gene...
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Article
Genetic Variation and Evolution of the Pi9 Blast Resistance Locus in the AA Genome Oryza Species
The rice nucleotide-binding site–leucine-rich repeat (NBS-LRR)-encoding resistance (R) gene Pi9 confers broad-spectrum resistance to the fungal pathogen Magnaporthe oryzae. The Pi9 locus comprises many NBS-LRR-li...
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Article
Manipulation of triose phosphate/phosphate translocator and cytosolic fructose-1,6-bisphosphatase, the key components in photosynthetic sucrose synthesis, enhances the source capacity of transgenic Arabidopsis plants
Photoassimilated carbons are converted to sucrose in green plant leaves and distributed to non-phototropic tissues to provide carbon and energy. In photosynthetic sucrose biosynthesis, the chloroplast envelope...
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Article
Iron homeostasis and fortification in rice
Iron (Fe) is an important micronutrient used by all living organisms for proper development. A deficiency in that element causes a metabolic imbalance that is deleterious to plant growth, making it a significa...
