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  1. Article

    Open Access

    Dual-color DNA-PAINT single-particle tracking enables extended studies of membrane protein interactions

    DNA-PAINT based single-particle tracking (DNA-PAINT-SPT) has recently significantly enhanced observation times in in vitro SPT experiments by overcoming the constraints of fluorophore photobleaching. However, ...

    Christian Niederauer, Chikim Nguyen, Miles Wang-Henderson in Nature Communications (2023)

  2. Article

    Open Access

    Tracking single particles for hours via continuous DNA-mediated fluorophore exchange

    Monitoring biomolecules in single-particle tracking experiments is typically achieved by employing fixed organic dyes or fluorescent fusion proteins linked to a target of interest. However, photobleaching typi...

    Florian Stehr, Johannes Stein, Julian Bauer, Christian Niederauer in Nature Communications (2021)

  3. Article

    Open Access

    Flat-top TIRF illumination boosts DNA-PAINT imaging and quantification

    Super-resolution (SR) techniques have extended the optical resolution down to a few nanometers. However, quantitative treatment of SR data remains challenging due to its complex dependence on a manifold of exp...

    Florian Stehr, Johannes Stein, Florian Schueder, Petra Schwille in Nature Communications (2019)