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Article
Open AccessDual-color DNA-PAINT single-particle tracking enables extended studies of membrane protein interactions
DNA-PAINT based single-particle tracking (DNA-PAINT-SPT) has recently significantly enhanced observation times in in vitro SPT experiments by overcoming the constraints of fluorophore photobleaching. However, ...
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Article
Open AccessTracking single particles for hours via continuous DNA-mediated fluorophore exchange
Monitoring biomolecules in single-particle tracking experiments is typically achieved by employing fixed organic dyes or fluorescent fusion proteins linked to a target of interest. However, photobleaching typi...
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Article
An order of magnitude faster DNA-PAINT imaging by optimized sequence design and buffer conditions
DNA points accumulation in nanoscale topography (DNA-PAINT) is a relatively easy-to-implement super-resolution technique. However, image acquisition is slow compared to most other approaches. Here, we overcome...
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Article
Open AccessFlat-top TIRF illumination boosts DNA-PAINT imaging and quantification
Super-resolution (SR) techniques have extended the optical resolution down to a few nanometers. However, quantitative treatment of SR data remains challenging due to its complex dependence on a manifold of exp...
