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Protocol
Arginine-Affinity Chromatography for Nucleic Acid (DNA and RNA) Isolation
Nucleic acid–based therapy has been emerging as a new strategy with great potential for the treatment of numerous diseases, especially those caused by gene defects. In this context, biotechnology plays a criti...
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Protocol
Conception of Plasmid DNA and Polyethylenimine Delivery Systems with Potential Application in DNA Vaccines Field
In DNA-based therapy research, the conception of a suitable vector to promote the target gene carriage, protection, and delivery to the cell is imperative. Exploring the interactions between polyethylenimine (...
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Protocol
Minicircle DNA Vaccine Purification and E7 Antigen Expression Assessment
Human papillomavirus (HPV ) has been extensively associated with the development of cervical due to the expression of like E7. This protein can interfere with pRB tumor suppressor activity, enabling the un...
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Article
Open AccessAptamer-based Targeted Delivery of a G-quadruplex Ligand in Cervical Cancer Cells
AS1411 is a G-rich DNA oligonucleotide that functions as an aptamer of the protein nucleolin, found at high levels on the surface of cancer cells but not on the surface of normal cells. Herein, we have studied...
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Article
Capto™ Resins for Chromatography of DNA: A Minor Difference in Ligand Composition Greatly Influences the Separation of Guanidyl-Containing Fragments
The differences in chromatographic behaviour of individual deoxynucleotides as well as small single-stranded and double-stranded DNA molecules have been examined for two resins from the Capto family: Capto Adhere
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Article
Advances in time course extracellular production of human pre-miR-29b from Rhodovulum sulfidophilum
The present study reports the successful production of human pre-miR-29b both intra- and extracellularly in the marine phototrophic bacterium Rhodovulum sulfidophilum using recombinant RNA technology. In a first ...
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Article
Open AccessRecombinant pre-miR-29b for Alzheimer´s disease therapeutics
MicroRNAs are arising as the next generation of diagnostic and therapeutic tools for gene silencing. Studies demonstrated that the miR-29 expression is decreased in Alzheimer’s disease (AD) patients displaying...
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Chapter
Layer-by-Layer Assembly for Biofunctionalization of Cellulosic Fibers with Emergent Antimicrobial Agents
Coating with polyelectrolyte multilayers has become a generic way to functionalize a variety of materials. In particular, the layer-by-layer (LbL) technique allows the coating of solid surfaces to give them se...
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Article
Open AccessAn artificial neural network for membrane-bound catechol-O-methyltransferase biosynthesis with Pichia pastoris methanol-induced cultures
Membrane proteins are important drug targets in many human diseases and gathering structural information regarding these proteins encourages the pharmaceutical industry ...
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Article
Folate-Targeted Multifunctional Amino Acid-Chitosan Nanoparticles for Improved Cancer Therapy
Tumor targeting nanomaterials have potential for improving the efficiency of anti-tumoral therapeutics. However, the evaluation of their biological performance remains highly challenging. In this study we desc...
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Protocol
p53-Encoding pDNA Purification by Affinity Chromatography for Cancer Therapy
The gene therapy approach based on reestablishment of p53 tumor suppressor, which acts as a prevailing guardian against malignant cell transformation, is raising new prospects on the outcome of an effective an...
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Article
Layer-by-Layer Deposition of Antibacterial Polyelectrolytes on Cotton Fibres
The introduction of molecules with biological properties on textile materials is essential for a number of biotechnological applications. With the purpose of testing new processes applied to textiles, in this ...
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Article
Plasmid DNA fermentation strategies: influence on plasmid stability and cell physiology
In order to provide sufficient pharmaceutical-grade plasmid DNA material, it is essential to gain a comprehensive knowledge of the bioprocesses involved; so, the development of protocols and techniques that al...
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Article
Screening nucleotide binding to amino acid-coated supports by surface plasmon resonance and nuclear magnetic resonance
Here, we describe a rapid and efficient screening method using surface plasmon resonance (SPR) and saturation transfer difference–nuclear magnetic resonance (STD-NMR) spectroscopy to yield information regardin...
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Article
Bacteriostatic versus bactericidal activity of ciprofloxacin in Escherichia coli assessed by flow cytometry using a novel far-red dye
As common microbiological methods for the assessment of bacteriostatic or bactericidal activities are very time-consuming, in this work we describe that the use of a novel far-red fluorescent stain, Vybrant Dy...
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Article
The Use of DRAQ5 to Monitor Intracellular DNA in Escherichia coli by Flow Cytometry
Flow cytometry provides a rapid and high-content multiparameter analysis of individual microorganisms within a population. In the past years, several fluorescent stains were developed in order to monitor DNA c...
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Article
Fractionation of Trichoderma Reesei Cellulases by Hydrophobic Interaction Chromatography on Phenyl-Sepharose
Trichoderma reesei cellulase complex was fractionated using hydrophobic interaction chromatography with a phenyl-Sepharose column. Using a linear gradient of ammonium sulphate in the eluent buffer, a selective s...
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Article
Kinetic study of the cellobiase activity of Trichoderma reesei cellulase complex at high substrate concentrations
At high cellobiose concentrations, the cellobiase activity of a Trichoderma reesei cellulase preparation does not follow Michaelis–Menten kinetics and shows substrate inhibition. Several rate equations were fi...
