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Protocol
E6 Tagged Protein Production, Extraction, and Purification from Escherichia coli Lysate
Cervical cancer has been extensively associated with human papillomavirus (HPV) due to the expression of oncoproteins such as E6. This protein can interfere with p53 tumor suppressor activity, blocking apoptos...
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Chapter
Nanotechnology to Correct Mitochondrial Disorders in Cancer Diseases
The correct functioning of mitochondria is essential for the balance and normal activity of the cell. Mutations in the genome of this organelle lead to the development of various severe pathologies, including ...
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Book
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Book
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Protocol
Conception of Plasmid DNA and Polyethylenimine Delivery Systems with Potential Application in DNA Vaccines Field
In DNA-based therapy research, the conception of a suitable vector to promote the target gene carriage, protection, and delivery to the cell is imperative. Exploring the interactions between polyethylenimine (...
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Protocol
Minicircle DNA Vaccine Purification and E7 Antigen Expression Assessment
Human papillomavirus (HPV ) has been extensively associated with the development of cervical due to the expression of like E7. This protein can interfere with pRB tumor suppressor activity, enabling the un...
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Protocol
p53-Encoding pDNA Purification by Affinity Chromatography for Cancer Therapy
The gene therapy approach based on reestablishment of p53 tumor suppressor, which acts as a prevailing guardian against malignant cell transformation, is raising new prospects on the outcome of an effective an...
