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Open AccessEstablishment and validation of a plasma oncofetal chondroitin sulfated proteoglycan for pan-cancer detection
Various biomarkers targeting cell-free DNA (cfDNA) and circulating proteins have been tested for pan-cancer detection. Oncofetal chondroitin sulfate (ofCS), which distinctively modifies proteoglycans (PGs) of ...
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Article
Open AccessChanges of CD103-expressing pulmonary CD4+ and CD8+ T cells in S. japonicum infected C57BL/6 mice
Recent studies have shown that CD103 is an important marker for tissue-resident memory T cells (TRM) which plays an important role in anti-infection. However, the role of CD103+ TRM was not elucidated in the prog...
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Open AccessPlasmodium vivax chloroquine resistance links to pvcrt transcription in a genetic cross
Mainstay treatment for Plasmodium vivax malaria has long relied on chloroquine (CQ) against blood-stage parasites plus primaquine against dormant liver-stage forms (hypnozoites), however drug resistance confronts...
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Open AccessA set of microsatellite markers to differentiate Plasmodium falciparum progeny of four genetic crosses
Four Plasmodium falciparum genetic crosses (HB3×3D7, HB3×Dd2, 7G8×GB4, and 803×GB4) have produced sets of recombinant progeny that are widely used for malaria research, including investigations of anti-malarial d...
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Open AccessMutations in the Plasmodium falciparum chloroquine resistance transporter, PfCRT, enlarge the parasite’s food vacuole and alter drug sensitivities
Mutations in the Plasmodium falciparum chloroquine resistance transporter, PfCRT, are the major determinant of chloroquine resistance in this lethal human malaria parasite. Here, we describe P. falciparum lines s...
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Open AccessPrevalence of Plasmodium falciparum anti-malarial resistance-associated polymorphisms in pfcrt, pfmdr1 and pfnhe1 in Muheza, Tanzania, prior to introduction of artemisinin combination therapy
A report of the chloroquine and amodiaquine resistance pfcrt-SVMNT haplotype in Tanzania raises concern about high-level resistance to the artesunate-amodiaquine combination treatment widely employed in Africa. M...
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PfSETvs methylation of histone H3K36 represses virulence genes in Plasmodium falciparum
The malaria parasite Plasmodium falciparum escapes immune detection by expressing one of 60 antigenically distinct var genes at any one time during the course of infection: here it is shown that the P. falciparum
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Open AccessMolecular and epidemiological characterization of Plasmodium vivax recurrent infections in southern Mexico
In southern Mexico, malaria transmission is low, seasonal, and persistent. Because many patients are affected by two or more malaria episodes caused by Plasmodium vivax, we carried out a study to determine the ti...
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Open AccessHigh recombination rates and hotspots in a Plasmodium falciparum genetic cross
The human malaria parasite Plasmodium falciparum survives pressures from the host immune system and antimalarial drugs by modifying its genome. Genetic recombination and nucleotide substitution are the two major ...
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Article
Open AccessLack of allele-specific efficacy of a bivalent AMA1 malaria vaccine
Extensive genetic diversity in vaccine antigens may contribute to the lack of efficacy of blood stage malaria vaccines. Apical membrane antigen-1 (AMA1) is a leading blood stage malaria vaccine candidate with ...
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Article
Plasmodium falciparum genome-wide scans for positive selection, recombination hot spots and resistance to antimalarial drugs
** of 189 culture-adapted Plasmodium falciparum parasites and examine population structure, selection and recombination hotspots. They also conduct genom...
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Article
Open AccessAnalysis of salivary transcripts and antigens of the sand fly Phlebotomus arabicus
Sand fly saliva plays an important role in blood feeding and Leishmania transmission as it was shown to increase parasite virulence. On the other hand, immunity to salivary components impedes the establishment of...
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Article
Open AccessDetection of genome-wide polymorphisms in the AT-rich Plasmodium falciparum genome using a high-density microarray
Genetic map** is a powerful method to identify mutations that cause drug resistance and other phenotypic changes in the human malaria parasite Plasmodium falciparum. For efficient map** of a target gene, it i...
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Open AccessInsight into the sialome of the castor bean tick, Ixodes ricinus
In recent years, there have been several sialome projects revealing transcripts expressed in the salivary glands of ticks, which are important vectors of several human diseases. Here, we focused on the sialome...
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Article
Open AccessThe midgut transcriptome of Lutzomyia longipalpis: comparative analysis of cDNA libraries from sugar-fed, blood-fed, post-digested and Leishmania infantum chagasi-infected sand flies
In the life cycle of Leishmania within the alimentary canal of sand flies the parasites have to survive the hostile environment of blood meal digestion, escape the blood bolus and attach to the midgut epitheli...
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Open AccesscDNA sequences reveal considerable gene prediction inaccuracy in the Plasmodium falciparum genome
The completion of the Plasmodium falciparum genome represents a milestone in malaria research. The genome sequence allows for the development of genome-wide approaches such as microarray and proteomics that will ...
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Genome-wide variation and identification of vaccine targets in the Plasmodium falciparum genome
One goal in sequencing the Plasmodium falciparum genome, the agent of the most lethal form of malaria, is to discover vaccine and drug targets1. However, identifying those targets in a genome in which ∼60% of gen...
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Correction: Chromosome-wide SNPs reveal an ancient origin for Plasmodium falciparum
Nature 418, 323–326 (2002). It has been drawn to our attention that the 3D7 parasite, labelled as an African parasite in our Letter, was initially isolated from The Netherlands, although we have some genetic e...
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Chromosome-wide SNPs reveal an ancient origin for Plasmodium falciparum
The Malaria's Eve hypothesis, proposing a severe recent population bottleneck (about 3,000–5,000 years ago) of the human malaria parasite Plasmodium falciparum, has prompted a debate about the origin and evolutio...
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Genetic diversity and chloroquine selective sweeps in Plasmodium falciparum
Widespread use of antimalarial agents can profoundly influence the evolution of the human malaria parasite Plasmodium falciparum. Recent selective sweeps for drug-resistant genotypes may have restricted the genet...
