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Article
Detection of cauliflower mosaic virus by the polymerase chain reaction: testing of food components for false-positive 35S-promoter screening results
Today DNA-based techniques are very common for the detection of genetically modified organisms (GMOs) in food products. For fast and easy detection of GMOs, polymerase chain reaction (PCR) screening methods, ...
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Article
A spelt-specific γ-gliadin gene: discovery and detection
Polymerase chain reaction (PCR) primers GAG5 and GAG6 were designed based on published γ-gliadin gene sequences and applied to 35 cultivars of closely related spelt (Triticum spelta L.) and hexaploid wheat (T. ae...
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Article
Improving PCR efficiency
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Article
Goitrin — a nitrosatable constituent of plant foodstuffs
N-nitrosamides are known as direct-acting carcinogens at the site of their formation; they do not need any metabolic activation in vivo. The conditions leading to their formation in the stomach, and also their...
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Article
Pyrrolizidine alkaloids fromSymphytum officinale L. and their percutaneous absorption in rats
An analysis of a commercial sample of Symphyti radix originating from Poland with a total alkaloid content of 0.07% revealed the presence of 7 pyrrolizidine alkaloid-N-oxides: 7-acetyl intermedine, 7-acetyl ly...
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Article
Stereology of liver biopsies from healthy volunteers
The stereologioal model and the base-line data of normal human liver needle biopsy-specimens are presented. Four reference systems were introduced: 1 cm3 of liver tissue, 1 cm3 of hepatoeyte, 1 cm3 of hepatocytic...
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Article
Fate of Chiral Acetates in the Citric Acid Cycle
The condensation of acetyl-CoA with oxaloacetate, catalysed by pig heart citrate synthase, takes place with inversion of configuration at the methyl group.
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Article
Asymmetric Methyl Groups: Preparation and Detection of Chiral Methyl Groups