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17 Result(s)

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  1. No Access

    Article

    In Vitro Glycoxidation of Insoluble Fibrous Type I Collagen: Solubilization and Advanced Glycation End Products

    The deleterious effects of glycoxidation are dependent on the half-life of proteins. Collagen, the main component of extracellular matrices, is a long live protein and thus may be sensitive to the glycoxidatio...

    M. Meli, R. Granouillet, E. Reynaud, A. Chamson, J. Frey… in Journal of Protein Chemistry (2003)

  2. No Access

    Article

    Changes in Glycation of Fibrous Type I Collagen During Long-Term In Vitro Incubation with Glucose

    The course of glycation of calf skin fibrous type I collagen was monitored in vitro under physiological conditions during an 8-week incubation period in order to take into account the long half-life of this prote...

    M. Meli, R. Granouillet, E. Reynaud, A. Chamson, J. Frey… in Journal of Protein Chemistry (2003)

  3. No Access

    Article

    Mineralization and Alkaline Phosphatase Activity in Collagen Lattices Populated by Human Osteoblasts

    Adult human osteoblastic cells were grown in a native type I collagen gel. Proliferation and viability analyses showed that cells rapidly stopped dividing and became blocked in the G0G1 phase (91% on day 13)....

    A. Rattner, O. Sabido, J. Le, L. Vico, C. Massoubre… in Calcified Tissue International (2000)

  4. No Access

    Article

    An improved scheme of leucine derivative fragmentation in mass spectrometry

    This study illustrates the contribution of stable isotopes to amino acid mass spectrometry. The mass spectra of natural leucine and 113C leucine were compared and the mass fragmentography pattern analysed. This a...

    C. Perier, J. Doumit, J. Frey in Amino Acids (1996)

  5. No Access

    Article

    Separation of amino acids using ion-paired reversed-phase high-performance liquid chromatography with special reference to collagen hydrolysate

    The collagen study includes the analysis of its characteristic amino acids: proline, hydroxyproline, lysine, hydroxylysine. HPLC offers an interesting device if associated with on-line radiometric detection fo...

    J. Frey, A. Chamson, N. Raby in Amino Acids (1993)

  6. No Access

    Article

    A direct fluorimetric DNA assay on cell suspensions

    An improved direct fluorimetric assay is described using bisbenzimidazol fluorescence at 356 nm excitation and 458 nm emission wavelengths. The turbidity of the medium was shown to have no effect on fluorescen...

    M. P. Bonis, N. Germain, J. Frey in Journal of tissue culture methods (1991)

  7. No Access

    Article

    Ω mutagenesis in gram-negative bacteria: A selectable DNA fragment which terminates transcription in a wide range of bacterial species

    J. Frey, J. Deshusses, H. M. Krisch in Experientia (1986)

  8. No Access

    Chapter

    Mutagenesis

    Bacterial plasmids are autonomously replicating mini chromosomes (replicons) which contain genetic information for many different properties, including the production of toxins and adhesion antigens, resistanc...

    R. Eichenlaub, A. Pühler, V. Krishnapillai, H. Heilmann… in Advanced Molecular Genetics (1984)

  9. No Access

    Chapter

    Basic Methods

    Gene transfer in bacteria occurs by three main processes: transformation and transfection, which involve the direct uptake of free DNA by bacteria; transduction, which involves bacteriophage mediated transmiss...

    M. P. O’Connell, U. Priefer, N. Datta, M. E. Nugent… in Advanced Molecular Genetics (1984)

  10. No Access

    Chapter

    Gene Cloning

    Genetic recombination is the fundamental biological process of exchange of genetic information between different chromosomes. Its constant activity during evolution has enabled the large number of spontaneousl...

    K. N. Timmis, G. Brady, J. Frey, A. Pühler, U. Priefer… in Advanced Molecular Genetics (1984)

  11. No Access

    Article

    In-vitro responses to ascorbate and manganese in fibroblasts from a patient with prolidase deficiency and iminodipeptiduria: Cell growth, prolidase activity and collagen metabolism

    After successful ascorbate and manganese treatment of a female patient with prolidase deficiency and iminodipeptiduria, we attempted to explain the mechanism of action of these drugsin vitro, using them preferent...

    I. Myara, C. Charpentier, C. Wolfrom, M. Gautier… in Journal of Inherited Metabolic Disease (1983)

  12. No Access

    Chapter and Conference Paper

    Catabolite Inactivation of Phosphoenolpyruvate Carboxykinase and Aminopeptidase I in Yeast

    Catabolite inactivation of yeast enzymes provides a typical example for the control of enzyme activity by selective inactivation. This inactivation has been defined by Holzer (1976) as the rapid irreversible l...

    M. Müller, J. Frey in Metabolic Interconversion of Enzymes 1980 (1981)

  13. No Access

    Article

    Place de la prolyl hydroxylase dans la synthèse du collagène de cellules KB

    KB cells, which synthetized collagen at a low rate, shown a prolyl hydroxylase activity at the same rate that fibroblast. The relationship between collagen synthesis and prolyl hydroxylase activity in these ce...

    J. Farjanel, P. Vallier, M. C. Ronzière, J. Frey in Experientia (1979)

  14. No Access

    Article

    The effects of an Escherichia coli dnaAts mutation on the replication of the plasmids colE1, pSC101, R100.1 and RTF-TC

    The rate of replication of the plasmids colE1, pSC101, R100.1 and pAR132 (an RTF-TC derivative of the drug resistance factor R100.1) has been investigated directly by DNA: DNA hybridization. These rates have b...

    J. Frey, M. Chandler, L. Caro in Molecular and General Genetics MGG (1979)

  15. Article

    Étude de l'activité de la protocollagène proline hydroxylase de foie d'embryon de Poulet en fonction de l'âge

    In chick embryo liver, there was a peak activity of protocollagen proline hydroxylase (PPH) on the 18th day of the embryonnic state. The effect of triton X100 suggested that PPH was a particulate enzyme, in co...

    Geneviève Helfre, J. Farjanel, Juliette Fonvielle, J. Frey in Experientia (1974)

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  16. No Access

    Article

    Étude biochimique des poissons: 1. Variations semestrielles de la teneur en eau et en protéines du tissu musculaire de la truite Arc-en-ciel (Salmo gairdnerii Rich)

    The authors have noted, during the reproductive cycle of the Rainbow trout (Salmo gairdnerii Rich), important variations concerning water and total proteins content. This study shows a great correlation between t...

    J. Gras, R. Reynaud, L. Gamoty, J. Frey, J. C. Henry in Experientia (1967)

  17. No Access

    Article

    Étude biochimique des poissons: 2. Variations semestrielles de la teneur en glycogène, en lipides totaux et en minéraux du tissu musculaire de la truite Arc-en-ciel (Salmo gairdnerii Rich)

    The variations of glycogen, lipids and ashes in muscular tissue of the Rainbow trout (Salmo gairdnerii Rich.) show that during sexual cycle there are 4 periods. Each period corresponds to a particular biological ...

    J. Gras, R. Reynaud, L. Gamoty, J. Frey, J. C. Henry in Experientia (1967)