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Article
Free surface flow between two horizontal concentric cylinders
Results are reported on a combined experimental and numerical investigation of a free surface flow at small Reynolds numbers. The flow is driven by the rotation of the inner of two horizontal concentric cylind...
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Article
The long lifespan and low turnover of human islet beta cells estimated by mathematical modelling of lipofuscin accumulation
Defects in pancreatic beta cell turnover are implicated in the pathogenesis of type 2 diabetes by genetic markers for diabetes. Decreased beta cell neogenesis could contribute to diabetes. The longevity and tu...
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Article
Amyloid fibril formation is progressive and correlates with beta-cell secretion in transgenic mouse isolated islets
Aims/hypothesis. Amyloid fibrils are formed in islets isolated from transgenic mice expressing the gene for human islet amyloid polypeptide (IAPP) by an unknown mechanism. This model of islet amy...
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Article
Fibrillar islet amyloid polypeptide (amylin) is internalised by macrophages but resists proteolytic degradation
Pancreatic islet amyloid, formed from islet amyloid polypeptide, is found in 96% of Type II (non-insulin-dependent) diabetic patients. Islet amyloidosis is progressive and apparently irreversible. Fibrils immu...
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Chapter and Conference Paper
Peptide-Secreting Dendrites: New Controls for Neuroendocrine Neurons
Peptidergic neurons are well known to synthesize and package peptides in their perikarya, and transport the peptide-containing dense-cored vesicles along their axons for release at the axon terminals. Immunocy...
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Article
Development of microglia and macrophages in the postnatal rat pituitary
Microglia and macrophages, immunolabelled with F4/80 (which binds a 160-kDa plasmalemmal glycoprotein) and OX-42 (which labels the complement type 3 receptor, CR3), were identified in the neuro- and adenohypop...
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Article
Synaptic relationship of substance P-like-immunoreactive nerve fibers with gland cells of the anterior pituitary in the rat
Our previous studies have proved the presence of substantial amounts of peptidergic nerve fibers in the anterior pituitary of human, monkey, dog, and rat, and synaptic contacts have been demonstrated on cortic...
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Chapter and Conference Paper
Hematopoietic Transcriptional Regulation by the Myeloid Zinc Finger Gene, MZF-1
Transcriptional regulators control much of hematopoiesis. One such transcriptional regulator is the myeloid zinc finger gene MZF-1. MZF-1 has been localized to the telomere of chromosome 19q, where a large num...
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Article
Immunophenotypic evidence for distinct populations of microglia in the rat hypothalamo-neurohypophysial system
The morphology, distribution and immunophenotype of microglia throughout the adult rat hypothalamo-neurohypophysial system was examined. Four macrophage-associated antibodies (OX-42, F4/80, ED1 and ED2) were u...
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Article
Chronic overproduction of islet amyloid polypeptide/amylin in transgenic mice: lysosomal localization of human islet amyloid polypeptide and lack of marked hyperglycaemia or hyperinsulinaemia
Type 2 (non-insulin-dependent) diabetes mellitus is characterised by hyperglycaemia, peripheral insulin resistance, impaired insulin secretion and pancreatic islet amyloid formation. The major constituent of i...
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Article
Localisation of islet amyloid polypeptide and its carboxy terminal flanking peptide in islets of diabetic man and monkey
Islet amyloid polypeptide is a normal constituent of islet Beta cells and is derived from a larger precursor by removal of flanking peptides at the carboxy (C) and amino (N) terminals. The role of these flanki...
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Article
Membrane routing during exocytosis and endocytosis in neuroendocrine neurones and endocrine cells: use of colloidal gold particles and immunocytochemical discrimination of membrane compartments
The hypothesis that the retrieval of membranes of neurohypophysial neurosecretory granules (NSG) and small electron-lucent microvesicles occurs by different routes was tested by incubating neurohypophysial neu...
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Chapter
Intracellular Formation of Amyloid Fibrils in B-Cells of Human Insulinoma and Pre-Diabetic Monkey Islets
Amyloid formed from islet amyloid polypeptide (lAPP) is present in pancreatic islets of spontaneously diabetic humans, monkeys and cats and in some insulinomas. The factors which cause amyloid deposition are u...
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Article
Ultrastructural characterisation of vasopressinergic terminals in the lateral septum of murine brains by use of monoclonal anti-neurophysins
Synapses in the lateral septum of the murine brain have been investigated by ultrastructural immunocytochemistry, using monoclonal anti-neurophysins in both immunoperoxidase and immunogold techniques. In the r...
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Article
Bone formation in organ cultures of bone marrow
Bone formation in organ cultures of intact marrow fragments from mouse is described. Marrow explants were cultured on the top surface of a millipore filter at a gas-liquid interface. Observations with both lig...
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Chapter
Anatomy and Function of the Classic Vasopressin-Secreting Hypothalamus-Neurohypophysial System
It is probably no exaggeration to declare that the hypothalamus-neurohypophysial system (HNS) is the quintessence of neurosecretion and vasopressin (VP) the quintessential brain peptide. The system comprises m...
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Article
Improved visualization of the immunoreactive hypothalamo-neurohypophysial system by use of immuno-gold techniques
Ultrastructural post-embedding immuno-gold techniques were applied to the supraoptic nucleus and the neurohypophysis of mice and rats. The primary antibodies were three different monoclonal antineurophysins, u...
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Article
Pancreatic glucagon in human foetal stomach
A combination of immunocytochemistry at light and electron microscopic levels, direct radioimmunoassay and measurement after gel chromatography have been used to identify and characterise a glucagon-like pepti...
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Article
A mutation affecting L-serine and energy metabolism in E. coli K12
The effects of a pleiotropic mutation ssd are described. This mutation results in decreased efficiency in the use of glucose and fructose as carbon source, inability to use succinate or to grow anaerobically, an ...
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Article
Calcium localization in the rat neurohypophysis
Neurosecretory nerve endings in the rat neurohypophysis release their hormones by exocytosis1 subsequent to an influx of calcium from the external medium2. The nerve endings are characterized by the presence of n...
