Plant Virology Protocols
New Approaches to Detect Viruses and Host Responses
Page
%P
-
Article
Open AccessTruncated yet functional viral protein produced via RNA polymerase slippage implies underestimated coding capacity of RNA viruses
RNA viruses use various strategies to condense their genetic information into small genomes. Potyviruses not only use the polyprotein strategy, but also embed an open reading frame, pipo, in the P3 cistron in the...
-
Book
-
Protocol
Construction of Infectious cDNA Clones Derived from the Potyviruses Clover Yellow Vein Virus and Bean Yellow Mosaic Virus
Infectious cDNA clones are now indispensible tools for the genetic analysis of viral factors involved in viral virulence and host resistance. In addition, infectious cDNA-derived virus vectors that express for...
-
Article
Characterization of the recessive resistance gene cyv1 of Pisum sativum against Clover yellow vein virus
Two recessive resistance genes against Clover yellow vein virus (ClYVV), cyv1 and cyv2, have been previously reported. We recently screened resistant peas from a separate set of pea lines and classified them into...
-
Article
Heterologous expression of viral suppressors of RNA silencing complements virulence of the HC-Pro mutant of clover yellow vein virus in pea
Many plant viruses encode suppressors of RNA silencing, including the helper component-proteinase (HC-Pro) of potyviruses. Our previous studies showed that a D-to-Y mutation at amino acid position 193 in HC-Pr...
-
Article
White clover mosaic virus-induced gene silencing in pea
Double-stranded RNAs formed in secondary structures and replicative intermediates of viral genomes are thought to strongly elicit RNA silencing. This phenomenon is known as virus-induced gene silencing (VIGS)....
-
Article
Open AccessScreening and analysis of genes expressed upon infection of broad bean with Clover yellow vein virus causing lethal necrosis
Clover yellow vein virus (ClYVV) causes lethal systemic necrosis in legumes, including broad bean (Vicia faba) and pea (Pisum sativum). To identify host genes involved in necrotic symptom expression after ClYVV i...
-
Article
What can we learn from basic plant virology?
-
Article
The cyv-2 resistance to Clover yellow vein virus in pea is controlled by the eukaryotic initiation factor 4E
The same mutant allele of eukaryotic initiation factor 4E (eIF4E) that confers resistance to Pea seed-borne mosaic virus (sbm-1) and the white lupine strain of Bean yellow mosaic virus (wlv) also confers resistan...
-
Article
Quick and timely publication in the Journal of General Plant Pathology
-
Article
Genetic analysis of lethal tip necrosis induced by Clover yellow vein virus infection in pea
Clover yellow vein virus (ClYVV) elicits lethal tip necrosis in the pea line PI 118501. Pea line PI 118501 develops necrotic lesions and veinal necrosis on inoculated leaves, followed by systemic necrosis, leadin...
-
Article
Open AccessReconstruction of putative DNA virus from endogenous rice tungro bacilliform virus-like sequences in the rice genome: implications for integration and evolution
Plant genomes contain various kinds of repetitive sequences such as transposable elements, microsatellites, tandem repeats and virus-like sequences. Most of them, with the exception of virus-like sequences, do...
-
Article
Positional effect of gene insertion on genetic stability of a clover yellow vein virus-based expression vector
The stability of the inserted genes in the viral expression vector varied depending on the sequence introduced and the position of insertion. Infectious cDNA to Clover yellow vein virus (pClYVV) was modified to i...
-
Article
Genetic Studies on Rice dwarf virus
-
Article
Complete Nucleotide Sequence of the Rice Tungro Spherical Virus Genome of the Highly Virulent Strain Vt6
The complete nucleotide sequence of rice tungro spherical virus (RTSV) strain Vt6, originally from Mindanao, the Philippines, with higher virulence to resistant rice cultivars, was determined and compared with...
-
Protocol
Reovirus Isolation and RNA Extraction
Plant reoviruses are classified in three genera in the family Reoviridae: Phytoreovirus, Fijivirus, and Oryzavirus. Fifteen viruses, including possible members, are described (1). With two exceptions, all of them...
-
Article
A cDNA Clone to Clover Yellow Vein Potyvirus Genome is Highly Infectious
We obtained a highly infectious cDNA clone of clover yellow vein virus (ClYVV). The cDNA fragments, from which a full-length cDNA clone was constructed, were sequenced, and the complete nucleotide sequence of ...
