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Article
Open AccessHippocampal and orbitofrontal neurons contribute to complementary aspects of associative structure
The ability to establish associations between environmental stimuli is fundamental for higher-order brain functions like state inference and generalization. Both the hippocampus and orbitofrontal cortex (OFC) ...
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Protocol
T Cell Receptor Repertoire Sequencing
The status of T cell receptors (TCRs) repertoire is associated with the occurrence and progress of various diseases and can be used in monitoring the immune responses, predicting the prognosis of disease and o...
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Article
Open AccessMetabolome and transcriptome analyses reveal quality change in the orange-rooted Salvia miltiorrhiza (Danshen) from cultivated field
The dry root and rhizome of Salvia miltiorrhiza Bunge, or Danshen, is a well-known, traditional Chinese medicine. Tanshinones are active compounds that accumulate in the periderm, resulting in red-colored roots. ...
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Article
An integrated strategy to identify genes responsible for sesquiterpene biosynthesis in turmeric
Metabolic module, gene expression pattern and PLS modeling were integrated to precisely identify the terpene synthase responsible for sesquiterpene formation. Functional characterization confirmed the feasibil...
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Article
Open AccessGenetic engineering of medium-chain-length fatty acid synthesis in Dunaliella tertiolecta for improved biodiesel production
Genetic engineering of microalgae to accumulate high levels of medium-chain-length fatty acids (MCFAs) represents an attractive strategy to improve the quality of microalgae-based biodiesel, but it has thus fa...
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Article
Open AccessNitrogen-induced metabolic changes and molecular determinants of carbon allocation in Dunaliella tertiolecta
Certain species of microalgae are natural accumulators of lipids, while others are more inclined to store starch. However, what governs the preference to store lipids or starch is not well understood. In this ...
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Article
Treatment of the distal fracture in radioulna based on the volar wrist dual channel approach and postoperative X-ray diagnosis
The fracture of the distal ulna and radius is a kind of fracture that results in high morbidity and occurrence rate and contributes to about one-sixth of the entire body’s fracture. In this study, we implement...
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Article
Open AccessRNA-Seq transcriptomic analysis with Bag2D software identifies key pathways enhancing lipid yield in a high lipid-producing mutant of the non-model green alga Dunaliella tertiolecta
For many years, increasing demands for fossil fuels have met with limited supply. As a potential substitute and renewable source of biofuel feedstock, microalgae have received significant attention. However, f...
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Article
An RNA polymerase II- and AGO4-associated protein acts in RNA-directed DNA methylation
Small interfering RNAs (siRNAs) are important regulators of gene expression in the plant model Arabidopsis, and intriguingly the vast majority of the small RNAs involved are generated from methylated regions of t...
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Article
Functional analysis of cis-acting sequences regulating root-specific expression in transgenic tobacco
Two different length fragments, RSF1 and RSF2 which contained the cis-acting sequences of root-specific gene TobRB7, were isolated from tobacco genome. The abilities of these fragments to direct root-specific exp...
