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Article
Open AccessClinicodemographic correlates of psychotic features in bipolar disorder – a multicenter study in China
Psychotic symptoms are prevalent in patients with bipolar disorder (BD). However, nearly all previous studies on differences in sociodemographic and clinical factors between patients with (BD P +) and without ...
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Article
Influenza A virus upregulates PRPF8 gene expression to increase virus production
A ddRT-PCR analysis was performed to detect cellular genes that are differentially expressed after influenza A virus (H1N1) infection of A549 cells. After ddRT-PCR, eight DNA fragments were identified. PRPF8, ...
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Article
Open AccessDuration of untreated bipolar disorder: a multicenter study
Little is known about the demographic and clinical differences between short and long duration of untreated bipolar disorder (DUB) in Chinese patients. This study examined the demographic and clinical features...
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Protocol
Studying Coronavirus–Host Protein Interactions
To understand the molecular mechanisms of viral replication and pathogenesis, it is necessary to establish the virus–host protein interaction networks. The yeast two-hybrid system is a powerful proteomic appro...
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Article
Open AccessDecreased plasma neuroactive amino acids and increased nitric oxide levels in melancholic major depressive disorder
Amino acid neurotransmitters and nitric oxide (NO) are involved in the pathogenesis of major depressive disorder (MDD). Here we want to establish whether changes in their plasma levels may serve as biomarker f...
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Article
SARS-CoV nucleocapsid protein interacts with cellular pyruvate kinase protein and inhibits its activity
The pathogenesis of SARS-CoV remains largely unknown. To study the function of the SARS-CoV nucleocapsid protein, we have conducted a yeast two-hybrid screening experiment to identify cellular proteins that ma...
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Article
Open AccessEnterovirus type 71 2A protease functions as a transcriptional activator in yeast
Enterovirus type 71 (EV71) 2A protease exhibited strong transcriptional activity in yeast cells. The transcriptional activity of 2A protease was independent of its protease activity. EV71 2A protease retained ...
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Article
Open AccessIncreased ATP generation in the host cell is required for efficient vaccinia virus production
To search for cellular genes up-regulated by vaccinia virus (VV) infection, differential display-reverse transcription-polymerase chain reaction (ddRT-PCR) assays were used to examine the expression of mRNAs f...
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Article
Expression and membrane integration of SARS-CoV E protein and its interaction with M protein
The severe acute respiratory syndrome (SARS)-CoV E gene fragment was cloned and expressed as a recombinant protein fused with a myc tag at the N-terminus in vitro and in Vero E6 cells. Similar to other N-glyco...
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Article
Interactions between M protein and other structural proteins of severe, acute respiratory syndrome-associated coronavirus
Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) structural proteins (S, E, M, and NC) localize in different subcellular positions when expressed individually. However, SARS-CoV M protein is...
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Article
Expression and membrane integration of SARS-CoV M protein
SARS-CoV M gene fragment was cloned and expressed as a recombinant protein fused with a V5 tag at the C-terminus in Vero E6 cells. In addition to un-glycosylated and glycosylated proteins, one product with sma...
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Article
Imbalanced free radicals and antioxidant defense systems in schizophrenia: A comparative study
Objective: To examine changes of blood oxidative-antiovidative level in schizophrenic patients and its relationship with clinical symptoms. Methods: Forty-six Chinese patients met DSM-IV (Diagnostic and Statis...
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Article
Metabolic profiling of the Arabidopsis pkl mutant reveals selective derepression of embryonic traits
Embryos express several unique differentiation characteristics, including the accumulation of a number of metabolites that are generally considered to be unique to seeds. PICKLE (PKL) codes for a CHD3-chromatin r...
