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Article
Optimization Design of the Multi-Layer Cross-Sectional Layout of An Umbilical Based on the GA-GLM
Marine umbilical is one of the key equipment for subsea oil and gas exploitation, which is usually integrated by a great number of different functional components with multi-layers. The layout of these compone...
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Article
Open AccessLiquid-liquid extraction intensification by micro-droplet rotation in a hydrocyclone
The previous literature reports that using a hydrocyclone as an extractor intensifies the mass transfer and largely reduces the consumption of extractant from 1800–2000 kg h−1 to 30–90 kg h−1. However, the intens...
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Article
Open AccessIn situ analysis of dynamic laminar flow extraction using surface-enhanced Raman spectroscopy
In this study, we performed micro-scale dynamic laminar flow extraction and site-specific in situ chloride concentration measurements. Surface-enhanced Raman spectroscopy was utilized to investigate the diffusion...
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Article
Open AccessMicrofluidic-based fabrication, characterization and magnetic functionalization of microparticles with novel internal anisotropic structure
Easy fabrication and independent control of the internal and external morphologies of core-shell microparticles still remain challenging. Core-shell microparticle comprised of a previously unknown internal ani...
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Article
Developments of subunit and VLP vaccines against influenza a virus
Influenza virus is a continuous and severe global threat to mankind. The continuously re-emerging disease gives rise to thousands of deaths and enormous economic losses each year, which emphasizes the urgency ...
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Article
Genetic modification of baculovirus expression vectors
As a protein expression vector, the baculovirus demonstrates many advantages over other vectors. With the development of biotechnology, baculoviral vectors have been genetically modified to facilitate high lev...
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Article
Production of CCHF virus-like particle by a baculovirus-insect cell expression system
Crimean-Congo Haemorrhagic Fever Virus (CCHFV) is a tick-born virus of the Nairovirus genus within the Bunyaviridae family, which is widespread and causes high fatality. The nucleocapsid of CCHFV is comprised of ...
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Article
Serial expression of the truncated fragments of the nucleocapsid protein of CCHFV and identification of the epitope region
The Crimean-congo hemorrhagic fever virus (CCHFV) is a geographically widespread fatal pathogen. Identification of the epitope regions of the virus is important for the diagnosis and epidemiological studies of...
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Article
Subglottic adenoid cystic carcinoma mistaken for asthma
Adenoid cystic carcinoma rarely occurs within the subglottic larynx. In this study, a case of subglottic adenoid cystic carcinoma was reported. A 54 year-old Chinese woman developed a sudden onset of chest dis...
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Article
Virion proteomics of large DNA viruses
Large DNA viruses normally have complex structures with many of protein components derived from both viral and host origins. The development in proteomics, especially mass spectrometry identification technique...
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Article
Evaluation of sensitivities and specificities of SARS-CoV detection by real-time quantitative reverse transcription-PCR assays
The etiological agent of severe acute respiratory syndrome (SARS) was identified as a new coronavirus, termed SARS-CoV. Establishment of an efficient and sensitive diagnostic system of SARS-CoV genetic materia...
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Article
An improved culture system for virus isolation and detection
Cell culture plays an important role in virology. It provides a platform for the detection and isolation of viruses as well as for the biochemistry and molecular biology based studies of viruses. In the presen...
