Abstract
The Crimean-congo hemorrhagic fever virus (CCHFV) is a geographically widespread fatal pathogen. Identification of the epitope regions of the virus is important for the diagnosis and epidemiological studies of CCHFV infections. In this study, expression vectors carrying series truncated fragments of the NP (nucleocapsid protein) gene from the S fragment of CCHFV strain YL04057 were constructed. The recombinant proteins were expressed in E.coli and purified for detection. The antigenic of the truncated fragments of NP was detected with a polyclonal serum (rabbit) and 2 monoclonal (mAbs) (14B7 and 43E5) against CCHFV by Western-blot analyses. The results showed that the three expressed constructs, which all contained the region 235AA to 305AA could be detected by mAbs polyclonal serum. The results suggest that region 235-305 aa of NP is a highly antigenic region and is highly conserved in the NP protein.
Article PDF
Similar content being viewed by others
Avoid common mistakes on your manuscript.
References
Calisher C H. 1996. History, classification and taxonomy of viruses in the family Bunyaviridae. In: The Bunyaviridae (Elliott R M. ed.), New York: Plenum Press, p1–17.
Deyde V M, Khristova M L, Rollin P E, et al. 2006. Crimean-Congo Hemorrhagic Fever Virus Genomics and Global Diversity. J Vriol, 80(17): 8834–8842.
Fack F, Hugle-Dorr B, Song D, et al. 1997. Epitope map** by phage display: Random versus gene-fragment libraries. J Immunol Metho, 206(1–2), 43–52.
Feng C H. 2004. Experimental Observation on Recognition of Viral Protein by Monoclonal Antibodies to XHF Virus. Endemic Diseases Bulletin, 19, 94–96.
Han L, Tang Q, Zhao X Q, et al. 2001. Serologic studies of **njiang hemorrhagic fever in Bachu county. Chin J Epidemiol, 23, 179–181.
Hoogstraal H. 1979. The epidemiology of tick borne Crimean-Congo hemorrhagic fever in Asia, Europe, and Africa. J Med Entomol, 15, 307–417.
Larsen J E, Lund O, Nielsen M. 2006. Improved method for predicting linear B-cell epitopes. Immunome Res, 24,2: 2.
Saijo M, Tang Q, Niikura M, et al. 2002. Recombinant Nucleoprotein-Based Enzyme-Linked Immunosorbent Assay for Detection of Immunoglobulin G Antibodies Crimean-Congo Hemorrhagic Fever Virus. J Clin MicroBiol, 40(5): 1587–1591.
Saijo M, Tang Q, Shimayi B, et al. 2005. Antigen-capture enzyme-linked immunosorbent assay for the diagnosis of crimean-congo hemorrhagic fever using a novel mono-clonal antibody. J Med Virol, 77: 83–88.
Schmaljohn C S, Hooper J W. 2001. Bunyaviridae: the viruses and their replication. In: Fields virology, 4th ed. Philadelphia: Lippincott-Raven Publishers, p1581–1602.
Shepherd A J, Swanepoel R, Shepherd S P, et al. 1985. A nosocomial outbreak of Crimean-Congo haemorrhagic fever at Tygerberg hospital. Part 5. Virological and serological observations. S Afr Med J, 68(10), 733–736.
Swanepoel R, Shepherd A J, Leman P A, et al. 1987. Epidemiological and clinical features of Crimean-Congo haemorrhagic fever in southern Africa. Am J Trop Med Hyg, 36(1), 120–132.
Author information
Authors and Affiliations
Corresponding authors
Additional information
Foundation items: National Key Technologies R & D Program of China during the 10th Five-Year Plan Period (2003BA712A08-03); The Knowledge Innovation Program of the Chinese Academy of Sciences (KSCX2-YW-N-065); The Foundation scientific and technological project from MOST (2007FY210700); The NSFC Grant (30860255).
Rights and permissions
About this article
Cite this article
Wei, Pf., Luo, Yj., Li, Tx. et al. Serial expression of the truncated fragments of the nucleocapsid protein of CCHFV and identification of the epitope region. Virol. Sin. 25, 45–51 (2010). https://doi.org/10.1007/s12250-010-3067-7
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s12250-010-3067-7