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Article
Minimum Cutsets for an Element of a Subspace Lattice over a Finite Vector Space
Let Ln(q) denote the lattice of subspaces of ann-dimensional vector space over the finite field of q elements, ordered byinclusion. In this note, we prove that for all n and m the minimum cutsetfor an element A w...
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Article
Two Kinds of Numbers and Their Applications
C. Radoux (J. Comput. Appl. Math. 115 (2000) 471–477) obtained a computational formula of Hankel determinants on some classical combinatorial sequences such as Catalan numbers and polynomials, Bel...
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Article
Molecular cloning of cDNAs for 14-3-3 and its protein interactions in a white-rot fungusPhanerochaete chrysosporium
The 14-3-3 is a highly conserved, ubiquitous expressed protein in eukaryotes and very important in the regulation of such crucial cellular processes as metabolism, signal transduction, cell-cycle control, apop...
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Article
Cloning and Expression of an α-Amylase Gene from Phanerochaete chrysosporium
Based on the genomic sequence and cDNA library screening, the cDNA sequence encoding an α-amylase was cloned from the filamentous white-rot fungus Phanerochaete chrysosporium and designated as pcamy1. Alignment r...
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Article
Construction of a novel cell-surface display system for heterologous gene expression in Escherichia coli by using an outer membrane protein of Zymomonas mobilis as anchor motif
A novel bacterial cell-surface display system was developed in Escherichia coli using omp1, a hypothetical outer membrane protein of Zymomonas mobilis. By using this system, we successfully expressed β-amylase ge...
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Article
Gene expression analysis of Phanerochaete chrysosporium during the transition time from primary growth to secondary metabolism
In order to identify the secondary metabolism-related genes of Phanerochaete chrysosporium growing under pure O2 and nitrogen-limited conditions, 2322 ESTs fragments originated from two suppression-subtractive li...
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Article
Substrate Specificity and Thermostability of the Dehairing Alkaline Protease from Bacillus pumilus
An alkaline protease (DHAP) from Bacillus pumilus has shown great potential in hide dehairing. To get better insights on its catalytic properties for application, the substrate specificity and thermostability wer...
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Article
Ethanol production by mixed-cultures ofPaenibacillus sp. andZymomonas mobilis using the raw starchy material from sweet potato
The conversion of raw starchy materials from sweet potato into ethanol in a mixed-culture of an amylolytic bacterium, Paenibacillus sp. and different strains of Zymomonas mobilis were studied. Raw starchy mate...
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Article
The alternative pathway in cucumber seedlings under low temperature stress was enhanced by salicylic acid
The alternative pathway is a cyanide-resistant and non-phosphorylatory electron transport pathway in mitochondria of higher plants. Alternative oxidase (AOX) is the terminal oxidase of this pathway. Our presen...
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Article
Open AccessClinical management of gastric cancer: results of a multicentre survey
The National Comprehensive Cancer Network clinical practice guidelines in oncology-gastric cancer guidelines have been widely used to provide appropriate recommendations for the treatment of patients with gast...
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Article
Changes in functional diversity of soil microbial community with addition of antibiotics sulfamethoxazole and chlortetracycline
Potential effects of antibiotics on agricultural soil microflora have recently become increasing concerns with antibiotic-contaminated biosolid now being used in agricultural land. However, changes of soil mic...
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Article
Plastid-signalling-mediated anthocyanin accumulation in mature Arabidopsis rosettes
Genetic and physiological studies have revealed evidences for multiple signaling pathways by which the plastid exerts retrograde control over photosynthesis-associated-nuclear-genes (PhANGs). Recently, more an...
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Article
A Simple and Reproducible Method for Directed Evolution: Combination of Random Mutation with dITP and DNA Fragmentation with Endonuclease V
An alternative method to combine mutagenesis PCR with dITP and fragmentation by endonuclease V for directed evolution was developed. In comparison to the routine protocol for directed evolution, dITP was used ...
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Article
Molecular cloning of grass carp (Ctenopharyngodon idellus) T-bet and GATA-3, and their expression profiles with IFN-γ in response to grass carp reovirus (GCRV) infection
Both T-bet and GATA-3, Th1/Th2 lineage-specific transcription factors, play important roles in the development of T cells and Th1/Th2 differentiation. In this study, T-bet and GATA-3 genes were cloned from gra...
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Article
Open AccessResearch on the relationship between fibrinogen level and subtypes of the TOAST criteria in the acute ischemic stroke
Cerebral infarction caused by different reasons seems differ in fibrinogen levels, so the current work intends to explore the relationship between the fibrinogen level and subtypes of the TOAST criteria in the...
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Article
The Chilli Veinal Mottle Virus Regulates Expression of the Tobacco Mosaic Virus Resistance Gene N and Jasmonic Acid/Ethylene Signaling Is Essential for Systemic Resistance Against Chilli Veinal Mottle Virus in Tobacco
Genetic, physiological, and molecular analyses have revealed that the stress-related phytohormones salicylic acid (SA), jasmonic acid (JA), and ethylene (ET) are known to participate in defense responses to mi...
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Article
Transcriptional analysis of adaptation to high glucose concentrations in Zymomonas mobilis
The ethanologenic bacterium Zymomonas mobilis is usually tolerant to high concentrations of glucose. The addition of sorbitol decreases the lag phase and increases ethanol yield and productivity of the bacteria i...
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Article
Studies on the Molecular Docking and Amino Acid Residues Involving in Recognition of Substrate in Proline Iminopeptidase by Site-Directed Mutagenesis
The proline iminopeptidase (PchPiPA) of Phanerochaete chrysosporium catalyze specifically hydrolysis of N-terminal proline from peptides. The substrate Pro-pNA was docked into the catalytic pocket and several ami...
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Article
Nitrate reductase-dependent nitric oxide production is required for regulation alternative oxidase pathway involved in the resistance to Cucumber mosaic virus infection in Arabidopsis
Nitrate reductase (NR), a key enzyme of nitrogen metabolism, catalyzes the reduction of nitrite to produce nitric oxide (NO). In this study, we investigated the role of NR-dependent NO in alleviating the cucumber...
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Article
Oleanolic acid alleviated pressure overload-induced cardiac remodeling
Previous study has demonstrated that oleanolic acid (OA) possessing the anti-inflammatory and anti-oxidant properties blunted high-glucose-induced diabetic cardiomyopathy and ameliorated experimental autoimmun...