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Article
Potential of rescue and reactivation of tumor suppressor p53 for cancer therapy
The tumor suppressor protein p53, a transcription product of the anti-oncogene TP53, is a critical factor in preventing cellular cancerization and killing cancer cells by inducing apoptosis. As a result, p53 is o...
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Article
Open AccessPresence of intrinsically disordered proteins can inhibit the nucleation phase of amyloid fibril formation of Aβ(1–42) in amino acid sequence independent manner
The molecular shield effect was studied for intrinsically disordered proteins (IDPs) that do not adopt compact and stable protein folds. IDPs are found among many stress-responsive gene products and cryoprotec...
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Chapter
Protein–Ligand Interactions Studied by NMR
Various solution NMR experiments for studying protein–ligand interactions have become indispensable techniques in both academia and industry. In general, solution NMR is superior to other physico-chemical meth...
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Article
Ecl1 is a zinc-binding protein involved in the zinc-limitation-dependent extension of chronological life span in fission yeast
Overexpression of Ecl1-family genes (ecl1 +, ecl2 +, and ecl3 +) results in the extension of the chronological life span in Schizosaccharomy...
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Article
1H, 13C, and 15N resonance assignment of the SPFH domain of human stomatin
Stomatin, a 288-residue protein, is a component of the membrane skeleton of red blood cells (RBCs), which helps to physically support the membrane and maintains its function. In RBCs, stomatin binds to the glu...
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Article
1H, 13C, and 15N resonance assignment of the first PDZ domain of mouse ZO-1
Zonula occludens-1 (ZO-1) is a scaffolding molecule critical to the formation of intercellular adhesion structures, such as tight junctions (TJs) and adherens junctions (AJs). ZO-1 contains three PDZ domains f...
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Article
A simplified recipe for assigning amide NMR signals using combinatorial 14N amino acid inverse-labeling
Assignment of backbone amide proton resonances is one of the most time-consuming stages of any protein NMR study when the protein samples behave non-ideally. A robust and convenient NMR procedure for analyzing...
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Article
1H, 13C, and 15N resonance assignment of the TIR domain of human MyD88
Myeloid differentiating factor 88 (MyD88) is one of a critical adaptor molecule in the Toll-like receptor (TLR) signaling pathway. The TIR domain of MyD88 serves as a protein–protein interaction module and int...
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Article
High-resolution multi-dimensional NMR spectroscopy of proteins in human cells
Until now, in-cell NMR spectroscopy of living cells has been limited to bacteria and Xenopus laevis oocytes. Wider application to living eukaryote cells has been limited by the relatively inefficient delivery of ...
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Article
Fine-tuning of protein domain boundary by minimizing potential coiled coil regions
Structural determination of individual protein domains isolated from multidomain proteins is a common approach in the post-genomic era. Novel and thus uncharacterized domains liberated from intact proteins oft...
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Article
In-cell NMR spectroscopy of proteins inside Xenopus laevis oocytes
In-cell NMR is an application of solution NMR that enables the investigation of protein conformations inside living cells. We have measured in-cell NMR spectra in oocytes from the African clawed frog Xenopus laev...
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Article
Crystal structure of thymine DNA glycosylase conjugated to SUMO-1
Members of the small ubiquitin-like modifier (SUMO) family can be covalently attached to the lysine residue of a target protein through an enzymatic pathway similar to that used in ubiquitin conjugation1, and are...
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Article
Letter to the Editor: 1H, 13C and 15N backbone resonance assignments of the N-terminal domain of Drosophila GCM protein
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Article
Solution structure of the PX domain, a target of the SH3 domain
The phox homology (PX) domain is a novel protein module containing a conserved proline-rich motif. We have shown that the PX domain isolated from the human p47phox protein, a soluble subunit of phagocyte NADPH ox...
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Article
Determination of the solution structure of the SH3 domain of human p56 Lck tyrosine kinase
The solution structure of the SH3 domain of human p56 Lck tyrosine kinase (Lck-SH3) has been determined by multidimensional heteronuclear NMR spectroscopy. The structure was calculated from a total of 935 expe...
