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1H, 13C, and 15N resonance assignment of the SPFH domain of human stomatin

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Abstract

Stomatin, a 288-residue protein, is a component of the membrane skeleton of red blood cells (RBCs), which helps to physically support the membrane and maintains its function. In RBCs, stomatin binds to the glucose transporter GLUT-1 and may regulate its function. Stomatin has a stomatin/prohibitin/flotillin/HflK (SPFH) domain at the center of its polypeptide chain. There are 12 SPFH domain-containing proteins, most of which are localized at the cellular or subcellular membranes. Although the molecular function of the SPFH domain has not yet been established, the domain may be involved in protein oligomerization. The SPFH domain of the archaeal stomatin homolog has been shown to form unique oligomers. Here we report the 15N, 13C, and 1H chemical shift assignments of the SPFH domain of human stomatin [hSTOM(SPFH)]. These may help in determining the structure of hSTOM(SPFH) in solution as well as in clarifying its involvement in protein oligomerization.

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Acknowledgments

This work was supported by Takeda Science Foundation and Suzuken Memorial Foundation. The authors are grateful to Mr. R. Taniguchi for his help in preparing the figure.

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Authors and Affiliations

  1. Division of Structural Biology, Graduate School of Medicine, Kobe University, 7-5-1, Kusunoki-cho, Chuo-ku, Kobe, Hyogo, 650-0017, Japan

    Tomoyuki Tsuruta, Natsuko Goda, Yoshitaka Umetsu, Naoko Iwaya, Yohta Kuwahara & Hidekazu Hiroaki

  2. The Structural Biology Research Center and Division of Biological Sciences, Graduate School of Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya, 464-8601, Japan

    Natsuko Goda, Yoshitaka Umetsu & Hidekazu Hiroaki

  3. Department of Molecular Engineering, Graduate School of Engineering, Kyoto University, Kyoto, 615-8510, Japan

    Naoko Iwaya

  4. Life BEANS (Bio Electromechanical Autonomous Nano Systems) Center, BEANS Project, Hachioji, Tokyo, 192-0982, Japan

    Yohta Kuwahara

  5. Global-COE (Center of Excellence) Program for Integrative Membrane Biology, Kobe University, Kobe, Japan

    Hidekazu Hiroaki

Authors
  1. Tomoyuki Tsuruta
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  2. Natsuko Goda
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  3. Yoshitaka Umetsu
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  4. Naoko Iwaya
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  5. Yohta Kuwahara
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  6. Hidekazu Hiroaki
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Correspondence to Hidekazu Hiroaki.

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Tsuruta, T., Goda, N., Umetsu, Y. et al. 1H, 13C, and 15N resonance assignment of the SPFH domain of human stomatin. Biomol NMR Assign 6, 23–25 (2012). https://doi.org/10.1007/s12104-011-9317-2

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  • DOI: https://doi.org/10.1007/s12104-011-9317-2

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