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  1. Article

    Open Access

    Bacterial cyclic diguanylate signaling networks sense temperature

    Many bacteria use the second messenger cyclic diguanylate (c-di-GMP) to control motility, biofilm production and virulence. Here, we identify a thermosensory diguanylate cyclase (TdcA) that modulates temperatu...

    Henrik Almblad, Trevor E. Randall, Fanny Liu, Katherine Leblanc… in Nature Communications (2021)

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  2. No Access

    Protocol

    Measuring Cyclic Diguanylate (c-di-GMP)-Specific Phosphodiesterase Activity Using the MANT-c-di-GMP Assay

    The second messenger, cyclic diguanylate (c-di-GMP), regulates a variety of bacterial cellular and social behaviors. A key determinant of c-di-GMP levels in cells is its degradation by c-di-GMP-specific phosph...

    Dorit Eli, Trevor E. Randall, Henrik Almblad, Joe J. Harrison… in c-di-GMP Signaling (2017)

  3. No Access

    Article

    Precision-engineering the Pseudomonas aeruginosa genome with two-step allelic exchange

    Here, the authors describe genetically engineering the Pseudomonas genome by two-step allelic exchange. Suicide vector-encoded alleles are used to generate mutations by homologous recombination at the single base...

    Laura R Hmelo, Bradley R Borlee, Henrik Almblad, Michelle E Love… in Nature Protocols (2015)