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Article
Open AccessThe NarX-NarL two-component system regulates biofilm formation, natural product biosynthesis, and host-associated survival in Burkholderia pseudomallei
Burkholderia pseudomallei is a saprophytic bacterium endemic throughout the tropics causing severe disease in humans and animals. Environmental signals such as the accumulation of inorganic ions mediates the biof...
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Article
Open AccessIdentification of a PadR-type regulator essential for intracellular pathogenesis of Burkholderia pseudomallei
Burkholderia pseudomallei (Bp) is the causative agent of melioidosis, a disease endemic to the tropics. Melioidosis manifests in various ways ranging from acute skin lesions to pneumonia and, in rare cases, infec...
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Chapter
Cyclic di-GMP in Burkholderia spp.
Burkholderia spp. survive in diverse ecological niches in association with soil, plants, and animals. In these environments, some members of the Burkholderia spp. participate in beneficial interactions that promo...
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Article
Open AccessTranscriptional and post-transcriptional regulation of PenA β-lactamase in acquired Burkholderia pseudomallei β-lactam resistance
Therapy of Burkholderia pseudomallei acute infections is largely limited to a few β-lactam antibiotics such as ceftazidime or meropenem. Although relatively rare, resistance emergence during therapy leads to trea...
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Article
The Current Status of Extracellular Polymeric Substances Produced by Burkholderia pseudomallei
Extracellular polymeric substances (EPS) that are produced by Burkholderia pseudomallei consist of diverse structural components that serve equally diverse protective and antigenic functions. B. pseudomallei is a...
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Protocol
Cyclic di-GMP-Responsive Transcriptional Reporter Bioassays in Pseudomonas aeruginosa
3′,5′-cyclic diguanosine monophosphate (cyclic di-GMP) is a bacterial secondary messenger molecule that regulates many important cellular activities and behaviors, such as motility and biofilm formation. While...
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Article
Precision-engineering the Pseudomonas aeruginosa genome with two-step allelic exchange
Here, the authors describe genetically engineering the Pseudomonas genome by two-step allelic exchange. Suicide vector-encoded alleles are used to generate mutations by homologous recombination at the single base...
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Chapter
Different Methods for Culturing Biofilms In Vitro
The field of biofilm microbiology, while by no means new, has been experiencing significant “growing pains” as more and more researchers become involved. One of the underlying reasons is the lack of standardiz...
