The Cancer Degradome
Proteases and Cancer Biology
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19 Result(s)
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Gunilla Høyer-Hansen
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Book
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Chapter
The Plasminogen Activation System as a Source of Prognostic Markers in Cancer
The components of the plasminogen activation system including the urokinase plasminogen activator, uPA, its cellular receptor, uPAR, and its inhibitor, PAI-1, are involved in cancer invasion and metastasis and...
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Chapter
Experiments to Incorporate In — Vitro Synthesized Photosystem I Components into Thylakoids of the Barley Mutant viridis — zb 63
Photosystem I is one of four multisubunit complexes of thylakoid membranes involved in the photosynthetic electron transport. The PSI of barley contains eight polypeptides (1). Three of these polypeptides are ...
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Article
Probing in vitro translation products with monoclonal antibodies to chlorophylla/b-binding proteins of barley thylakoids
The polypeptides of the barley light-harvesting protein of photosystem I (LHCI) share certain epitopes. At least two of these common epitopes are present in chlorophylla/b-protein 1 (Chla/b-Pl-CP29), as shown by ...
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Article
Photosystem II and cytochromeb-559 in the stroma lamellae of barley chloroplasts
The presence of photosystem II polypeptides and photosystem II activity in unstacked regions of barley thylakoid membranes were investigated by immunological and spectroscopic techniques. Immunogold labelling ...
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Article
Monoclonal antibodies to hordein polypeptides
Monoclonal antibodies to hordein polypeptides have been produced and characterized. All antibodies reacted with more than one polypeptide, indicating that different hordein polypeptides have common epitopes. O...
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Article
Probing in vitro translation products with monoclonal antibodies to a 15.2 kD polypeptide subunit of photosystem I
Three monoclonal antibodies to the 15.2 kD polypeptide subunit of a barley photosystem I(PSI) particle have been characterized. Immune-blot assays showed that there are at least two different antigenic sites o...
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Article
Monoclonal antibodies used for the characterization of the two putative iron-sulphur centre proteins associated with photosystem I
Monoclonal antibodies have been produced to the two putative 18.3 and 15.2 kD iron-sulphur centre proteins in barley. When photosystem I particles, containing only chlorophylla-protein 1 and the 18.3 and 15.2 kD ...
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Chapter
Monoclonal Antibodies to Chlorophyll α-protein 1 in Barley
When the hybridoma technique (Köhler, Milstein 1975) is employed, purification of the antigen prior to immunization is not necessary to obtain monospecific antibodies, so called monoclonal antibodies. In the p...
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Article
An analysis of temperature and light sensitivity intigrina mutants of barley
Quantitative determinations of chlorophylls, carotenes and xanthophylls were carried out by high pressure liquid chromatography on the nuclear gene mutanttigrina-o 34. In the dark the mutant accum...
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Chapter
The Use of Chloroplast Proteins in Crop Improvement
Detailed knowledge of the structure and function of the proteins involved in photosynthetic reactions can provide information to what extent these proteins can be modified in the course of crop improvement. Th...
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Article
Unstable chloroplast ribosomes in the cold-sensitive barley mutanttigrina-o 34
When grown at 20 °C the recessive nuclear gene mutanttigrina-o 34 in barley is deficient in 70S chloroplastic ribosomes, but contains wild-type levels of 80S cytoplasmic ribosomes. When grown at 3...
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Article
Characterization of six putative photosystem I mutants in barley
Six putative photosystem I mutants in five different nuclear genes of barley have been characterized with respect to their photosynthetic electron transport capabilities,P700, cytochromes, chlorophyll-proteins an...
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Article
Identification of haem-proteins in thylakoid polypeptide patterns of barley
Thylakoid polypeptides from barley were separated by polyacrylamide gel electrophoresis by use of either SDS or LiDS as the detergent. Staining of either gel-type with 3,3′,5,5′-tetramethylbenzidine-H2O2 revealed...
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Article
A photosystem I mutant in barley (Hordeum vulgare L.)
The nuclear gene mutantviridis-n 34 in barley has been characterized as a photosystem I mutant. The slow component of the light dependent absorption change at 518 nm and the photooxidation of cyto...
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Article
Indentification of coupling factor subunits in thylakoid polypeptide patterns of wild-type and mutant barley thylakoids using crossed immunoelectrophoresis
The extrinsic part of coupling factor (CF1) has been isolated from chloroplasts of barley and antibodies have been raised against the purified protein. The five different subunits of CF1 were identified among the...
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Article
Freeze-fracture evidence for the isolation of inside-out spinach thylakoid vesicles
An aqueous dextran/polyethylene glycol two phase system is used to separate spinach chloroplast thylakoid vesicles obtained by Yeda press treatment of a grana-enriched fraction. It is shown by freeze-fracturin...
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Article
Correlation between the freeze fracture appearance and polypeptide composition of thylakoid membranes in barley
Thylakoids isolated and purified from wild-type barley chloroplasts were treated to remove polypeptides without destroying the membrane structure. The polypeptide compositions of such basic membranes were dete...
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Article
Changes in the polypeptide composition of internal membranes of barley plastids during greening
A method is described for obtaining good yields of purified internal membranes of plastids from greening barley seedlings. The procedure is suitable, without modification, for isolating intact plastids from et...
