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Article
Open AccessCustomizable and stable multilocus chromosomal integration: a novel glucose-dependent selection system in Aureobasidium spp.
Non-conventional yeasts hold significant potential as biorefinery cell factories for microbial bioproduction. Currently, gene editing systems used for these yeasts rely on antibiotic and auxotrophic selection ...
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Article
Mechanism of cryogenic, solid solution and aging compound heat treatment of die-cast Al alloys considering microstructure variation
To develop a new compound heat treatment process for improving the mechanical properties of die-cast Al alloys, this study investigated the effects of cryogenic, solution and aging compound treatment on the mi...
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Article
Novel chromosomes and genomes provide new insights into evolution and adaptation of the whole genome duplicated yeast-like fungus TN3-1 isolated from natural honey
Aureobasidium melanogenum TN3-1 strain and A. melanogenum P16 strain were isolated from the natural honey and the mangrove ecosystem, respectively. The former can produce much higher pullulan from high concentra...
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Article
Genome-Wide Editing Provides Insights into Role of Unsaturated fatty Acids in Low Temperature Growth of the Psychrotrophic Yeast Metschnikowia bicuspidata var. australis W7-5
In order to know the function of C18:2 and C18:3 fatty acids in the cold growth of the psychrotrophic yeast M. bicuspidata var. australis W7-5, the mutant 1 without C18:2 fatty acid and the mutant 2 without C18:3
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Article
Open AccessBioconversion of non-food corn biomass to polyol esters of fatty acid and single-cell oils
Lignocellulose is a valuable carbon source for the production of biofuels and biochemicals, thus having the potential to substitute fossil resources. Consolidated bio-saccharification (CBS) is a whole-cell-bas...
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Article
Massoia Lactone Displays Strong Antifungal Property Against Many Crop Pathogens and Its Potential Application
Massoia lactone could be released from liamocins produced by Aureobasidium melanogenum M39. The obtained Massoia lactone was very stable and highly active against many fungal crop pathogens which cause many plant...
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Article
Liamocin overproduction by the mutants of Aureobasidium melanogenum 9–1 for effectively killing spores of the pathogenic fungi from diseased human skin by Massoia lactone
Liamocins and Massoia lactone have many applications. In this study, the glucose-derepressed mutant Δcrea5 in which the CREA gene was removed could produce 36.5 g/L of liamocins. Furthermore, overexpression of th...
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Article
Effects of laser shock processing, solid solution and aging, and cryogenic treatments on microstructure and thermal fatigue performance of ZCuAl10Fe3Mn2 alloy
The materials used in variable temperature conditions are required to have excellent thermal fatigue performance. The effects of laser shock processing (LSP), solid solution and aging treatment (T6), and cryog...
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Article
In situ synchrotron X-ray diffraction analysis of deformation behavior of a Nb/NiTi composite for biomedical applications
The deformation behavior involved in Nb/NiTi composite for biomedical applications within a large macroscopic strain range was investigated by tensile loading–unloading test and in situ synchrotron X-ray diffr...
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Article
The Genome-Wide Mutation Shows the Importance of Cell Wall Integrity in Growth of the Psychrophilic Yeast Metschnikowia australis W7-5 at Different Temperatures
In this study, it was found that a Cre/loxP system could be successfully used as a tool for editing the genome of the psychrophilic yeast Metschnikowia australis W7-5 isolated from Antarctica. The deletion and ov...
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Article
Melanin biosynthesis in the desert-derived Aureobasidium melanogenum XJ5-1 is controlled mainly by the CWI signal pathway via a transcriptional activator Cmr1
The melanin produced by Aureobasidium melanogenum XJ5-1 obtained from the Taklimakan Desert can play an important role in adaptation of the yeast strain to various stress treatments. It is very important to know ...
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Article
Open AccessGenome sequencing of a yeast-like fungal strain P6, a novel species of Aureobasidium spp.: insights into its taxonomy, evolution, and biotechnological potentials
This study aimed to look insights into taxonomy, evolution, and biotechnological potentials of a yeast-like fungal strain P6 isolated from a mangrove ecosystem.
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Article
Inulinase hyperproduction by Kluyveromyces marxianus through codon optimization, selection of the promoter, and high-cell-density fermentation for efficient inulin hydrolysis
This study aimed to overexpress an inulinase gene in Kluyveromyces marxianus to achieve the inulinase overproduction and preparation of ultra-high-fructose syrup. First, the inulinase gene (INU1 gene) was overexp...
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Article
Genome sequencing of Aureobasidium pullulans P25 and overexpression of a glucose oxidase gene for hyper-production of Ca2+-gluconic acid
Gluconic acid (GA) has many applications such as in the food and pharmaceutical industry. Aureobasidium pullulans P25 strain is able to produce high levels of Ca2+-GA. The genome length, GC content and the gene n...
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Article
Biosynthesis of some organic acids and lipids in industrially important microorganisms is promoted by pyruvate carboxylases
Pyruvate carboxylase (Pyc) catalyzes formation of oxaloacetic acid from pyruvic acid by fixing one mole of CO2. Many evidences have confirmed that biosynthesis of some different kinds of organic acids and intrace...
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Article
Production, Gene Cloning, and Overexpression of a Laccase in the Marine-Derived Yeast Aureobasidium melanogenum Strain 11-1 and Characterization of the Recombinant Laccase
Aureobasidium melanogenum strain 11-1 with a high laccase activity was isolated from a mangrove ecosystem. Under the optimal conditions, the 11-1 strain yielded the highest laccase activity up to 3120.0 ± 170 mU/...
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Article
Cloning, deletion, and overexpression of a glucose oxidase gene in Aureobasidium sp. P6 for Ca2+-gluconic acid overproduction
This study aimed to overexpress a glucose oxidase gene (GOD1) in Aureobasidium sp. P6 to achieve Ca2+-gluconic acid (GA) overproduction. The GOD1 gene was cloned, deleted, and overexpressed. A protein deduced fro...
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Article
Genetics of trehalose biosynthesis in desert-derived Aureobasidium melanogenum and role of trehalose in the adaptation of the yeast to extreme environments
Melanin plays an important role in the stress adaptation of Aureobasidium melanogenum XJ5-1 isolated from the Taklimakan desert. A trehalose-6-phosphate synthase gene (TPS1 gene) was cloned from K5, characterized...
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Article
CreA is directly involved in pullulan biosynthesis and regulation of Aureobasidium melanogenum P16
Aureobasidium melanogenum P16 is a high pullulan-producing yeast. However, glucose repression on its pullulan biosynthesis must be relieved. After the gene encoding a glucose repressor wa...
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Article
Surface decarburization behavior and its adverse effects of air-cooled forging steel C70S6 for fracture splitting connecting rod
Surface decarburization behavior and its adverse effects of air-cooled forging steel C70S6 for automobile engine fracture splitting connecting rod were investigated comprehensively by mechanical properties, mi...
